159405

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 249227..250026 Replicon chromosome
Accession NZ_CP054345
Organism Escherichia coli strain SCU-176

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HPE52_RS01225 Protein ID WP_000347251.1
Coordinates 249227..249691 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1PPV5
Locus tag HPE52_RS01230 Protein ID WP_001296435.1
Coordinates 249691..250026 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPE52_RS01195 244228..244662 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HPE52_RS01200 244680..245558 - 879 WP_001298314.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HPE52_RS01205 245548..246327 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HPE52_RS01210 246338..246811 - 474 WP_001298322.1 PTS N-acetylgalactosamine transporter subunit IIB -
HPE52_RS01215 246834..248114 - 1281 WP_000681931.1 tagatose-bisphosphate aldolase subunit KbaZ -
HPE52_RS01220 248363..249172 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HPE52_RS01225 249227..249691 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HPE52_RS01230 249691..250026 - 336 WP_001296435.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HPE52_RS01235 250175..251746 - 1572 WP_001539800.1 galactarate dehydratase -
HPE52_RS01240 252121..253455 + 1335 WP_074458634.1 galactarate/glucarate/glycerate transporter GarP -
HPE52_RS01245 253471..254241 + 771 WP_001058223.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T159405 WP_000347251.1 NZ_CP054345:c249691-249227 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T159405 NZ_CP070041:c2661576-2661403 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12321.92 Da        Isoelectric Point: 4.5392

>AT159405 WP_001296435.1 NZ_CP054345:c250026-249691 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159405 NZ_CP070041:c2661900-2661604 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB S1PPV5

References