Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 51988..52645 Replicon plasmid pMS14393B
Accession NZ_CP054305
Organism Klebsiella pneumoniae strain MS14393

Toxin (Protein)


Gene name higB Uniprot ID U3PDC3
Locus tag MS14393_RS28345 Protein ID WP_000270043.1
Coordinates 51988..52338 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag MS14393_RS28350 Protein ID WP_000124640.1
Coordinates 52343..52645 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS14393_RS28310 48462..48959 - 498 WP_000062185.1 hypothetical protein -
MS14393_RS28315 48962..49450 - 489 WP_001273096.1 DUF1643 domain-containing protein -
MS14393_RS28320 49547..49882 + 336 WP_000683476.1 hypothetical protein -
MS14393_RS28325 49897..50367 - 471 WP_001281821.1 hypothetical protein -
MS14393_RS28330 50360..50731 - 372 WP_000516916.1 hypothetical protein -
MS14393_RS28335 50742..50936 - 195 WP_000343597.1 hypothetical protein -
MS14393_RS28340 51277..51825 - 549 WP_001061574.1 transcriptional regulator -
MS14393_RS28345 51988..52338 + 351 WP_000270043.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
MS14393_RS28350 52343..52645 + 303 WP_000124640.1 XRE family transcriptional regulator Antitoxin
MS14393_RS28355 52672..52965 - 294 WP_001239997.1 chromosome segregation protein ParM -
MS14393_RS28360 53053..53325 - 273 WP_001043047.1 HU family DNA-binding protein -
MS14393_RS28365 53383..53910 - 528 WP_001236377.1 thermonuclease family protein -
MS14393_RS28370 54141..54998 - 858 WP_001167032.1 hypothetical protein -
MS14393_RS28375 54985..55215 - 231 WP_000972663.1 hypothetical protein -
MS14393_RS28380 55215..55733 - 519 WP_000210756.1 nitrite reductase -
MS14393_RS28385 55730..56176 - 447 WP_000919345.1 Fe3+-siderophore ABC transporter permease -
MS14393_RS28390 56176..56535 - 360 WP_000422768.1 hypothetical protein -
MS14393_RS28395 56592..57020 - 429 WP_000591074.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid sul2 / floR / qnrS1 / catA2 / aph(3')-Ia / mph(A) / blaTEM-1B / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 - 1..125232 125232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

Cro/C1-type helix-turn-helix domain

(33-79)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13328.21 Da        Isoelectric Point: 5.2421

>T159397 WP_000270043.1 NZ_CP054305:51988-52338 [Klebsiella pneumoniae]
MWVIETTDTFDEWFDALDDTDRANVLASMMVLRDRGPMLSRPYADTVNGSSYSNMKELRVQSKGDPIRAFFAFDPKRKGI
LLCAGNKTGDEKRFYEVMIPIADREFAAHLDKLKKE

Download         Length: 351 bp

>T159397 NZ_CP070041:989709-989816 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCGTGATTTTCTGGCACGACCTGGCAGCACCGATCCTTGCGGGAATTATTACCGCAGCGAT
TGTTGGCTGGTGGCGTAGCCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11006.73 Da        Isoelectric Point: 7.2036

>AT159397 WP_000124640.1 NZ_CP054305:52343-52645 [Klebsiella pneumoniae]
MARTLDQMLATEKPEVVAKAQKAATEMLLNIHLAELRDRMNLTQGEIAASLGVRQPTVSEMEKPGRDLKLSSIKRYVEAS
GGKLRLDVELPDGTHYGFAV

Download         Length: 303 bp

>AT159397 NZ_CP070041:c989653-989592 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTGACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G8AFB9

Antitoxin

Source ID Structure

References