Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 4137876..4138462 Replicon chromosome
Accession NZ_CP054303
Organism Klebsiella pneumoniae strain MS14393

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag MS14393_RS20580 Protein ID WP_002920800.1
Coordinates 4137876..4138244 (-) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID W9B1V1
Locus tag MS14393_RS20585 Protein ID WP_004174006.1
Coordinates 4138241..4138462 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS14393_RS20560 4133379..4134449 - 1071 WP_004150074.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -
MS14393_RS20565 4134451..4135296 - 846 WP_004145129.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
MS14393_RS20570 4135293..4136180 - 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
MS14393_RS20575 4136287..4137603 - 1317 WP_042938715.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
MS14393_RS20580 4137876..4138244 - 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
MS14393_RS20585 4138241..4138462 - 222 WP_004174006.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
MS14393_RS20590 4138626..4139339 - 714 WP_004145133.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
MS14393_RS20595 4139341..4140108 - 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
MS14393_RS20600 4140105..4141382 - 1278 WP_004188292.1 branched chain amino acid ABC transporter permease LivM -
MS14393_RS20605 4141379..4142305 - 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4132642..4153229 20587


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-50)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T159391 WP_002920800.1 NZ_CP054303:c4138244-4137876 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T159391 NZ_CP070041:81333-81584 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8151.15 Da        Isoelectric Point: 4.7769

>AT159391 WP_004174006.1 NZ_CP054303:c4138462-4138241 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT159391 NZ_CP070041:81581-81835 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A5E5YJY7

References