Detailed information of TA system TA159373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1857345..1857916
• Replicon: chromosome
• Accession: NZ_CP054301
• Organism: Marinomonas primoryensis strain MPKMM3633

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MP3633_RS08480
• Protein ID: WP_176335220.1
• Coordinates: 1857345..1857623 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: -
• Locus tag: MP3633_RS08485
• Protein ID: WP_176335221.1
• Coordinates: 1857623..1857916 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MP3633_RS08465	1854678..1855136	+	459	WP_176335217.1	hypothetical protein	-
MP3633_RS08470	1855760..1856107	+	348	WP_176335218.1	hypothetical protein	-
MP3633_RS08475	1856580..1856957	+	378	WP_176335219.1	hypothetical protein	-
MP3633_RS08480	1857345..1857623	+	279	WP_176335220.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MP3633_RS08485	1857623..1857916	+	294	WP_176335221.1	HigA family addiction module antidote protein	Antitoxin
MP3633_RS08490	1858017..1858400	-	384	Protein_1667	IS110 family transposase	-
MP3633_RS08495	1858819..1859355	-	537	WP_112139134.1	DUF615 domain-containing protein	-
MP3633_RS08500	1859362..1860714	-	1353	WP_176335222.1	magnesium transporter	-
MP3633_RS08505	1860722..1860991	-	270	WP_112139130.1	HPr family phosphocarrier protein	-
MP3633_RS08510	1861002..1861451	-	450	WP_112139128.1	PTS IIA-like nitrogen regulatory protein PtsN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (19-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10653.14 Da
• Isoelectric Point: 9.4209

>T159373 WP_176335220.1 NZ_CP054301:1857345-1857623 [Marinomonas primoryensis]
MIKTFKHKGLKKYFETGSVSGIQAKHQRKIRMQLAAIDTAQEIEDINLPGFNLHPLKGNRDGIWSITVNGNWRITFEFVD
GNAYILNYEDYH

Nucleotide

• Length: 279 bp

>T159373 NZ_CP070026:c3924201-3923947 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10795.39 Da
• Isoelectric Point: 7.3567

>AT159373 WP_176335221.1 NZ_CP054301:1857623-1857916 [Marinomonas primoryensis]
MNMHNPPHPGEFIYDVYLEPAGFSCRFLAKQLDVASSTLSRVLKGQSAVSPEMALRLSKALGRTPESWLAMQDAYDLWQA
KQHLNLANVHPVNFAVA

Nucleotide

• Length: 294 bp

>AT159373 NZ_CP070026:c3924449-3924198 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA