Detailed information of TA system TA159371

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 386689..387260
• Replicon: chromosome
• Accession: NZ_CP054301
• Organism: Marinomonas primoryensis strain MPKMM3633

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: MP3633_RS01780
• Protein ID: WP_148830867.1
• Coordinates: 386982..387260 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: MP3633_RS01775
• Protein ID: WP_176334235.1
• Coordinates: 386689..386982 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MP3633_RS01760	381708..384923	+	3216	WP_176334233.1	carbamoyl-phosphate synthase large subunit	-
MP3633_RS01765	384923..385399	+	477	WP_112136128.1	transcription elongation factor GreA	-
MP3633_RS01770	385514..386650	+	1137	WP_176334234.1	Fic family protein	-
MP3633_RS01775	386689..386982	-	294	WP_176334235.1	HigA family addiction module antidote protein	Antitoxin
MP3633_RS01780	386982..387260	-	279	WP_148830867.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MP3633_RS01785	387474..387689	-	216	WP_176334236.1	addiction module protein	-
MP3633_RS01790	388128..390593	-	2466	WP_176334237.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	386689..399183	12494

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (3-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (22-66)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10591.06 Da
• Isoelectric Point: 9.3845

>T159371 WP_148830867.1 NZ_CP054301:c387260-386982 [Marinomonas primoryensis]
MIKSFKHKGLKKYFETGSASGIQAKHKSKLRMQLTAIDTAQEIDDINLPGFKLHTLKGDRDGIWSITVNGNWRITFEFID
GNAYILNYEDYH

Nucleotide

• Length: 279 bp

>T159371 NZ_CP070026:c3450979-3450692 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10939.49 Da
• Isoelectric Point: 6.9759

>AT159371 WP_176334235.1 NZ_CP054301:c386982-386689 [Marinomonas primoryensis]
MSMHNPPHPGEFIYDVYLEPSGLSCRYLAKQLDVASSTLNRVLKGQSAVSPEMALRLSKALGRTPESWLAMQDNYDLWHA
KQHLNLANVHEVNFAMT

Nucleotide

• Length: 294 bp

>AT159371 NZ_CP070026:c3451218-3450979 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA