Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2767793..2768395 Replicon chromosome
Accession NZ_CP054282
Organism Escherichia coli strain IDR1900005638-01-02

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag HS093_RS13075 Protein ID WP_000897305.1
Coordinates 2768084..2768395 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HS093_RS13070 Protein ID WP_000356397.1
Coordinates 2767793..2768083 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HS093_RS13045 2763738..2764640 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
HS093_RS13050 2764637..2765272 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HS093_RS13055 2765269..2766198 + 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
HS093_RS13060 2766528..2766770 - 243 WP_001086388.1 hypothetical protein -
HS093_RS13065 2766989..2767207 - 219 WP_001295676.1 ribbon-helix-helix domain-containing protein -
HS093_RS13070 2767793..2768083 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
HS093_RS13075 2768084..2768395 - 312 WP_000897305.1 hypothetical protein Toxin
HS093_RS13080 2768624..2769532 + 909 WP_001162704.1 alpha/beta hydrolase -
HS093_RS13085 2769596..2770537 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HS093_RS13090 2770582..2771019 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
HS093_RS13095 2771016..2771888 - 873 WP_000920762.1 virulence factor BrkB family protein -
HS093_RS13100 2771882..2772481 - 600 WP_001295269.1 glucose-1-phosphatase -
HS093_RS13105 2772580..2773365 - 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T159326 WP_000897305.1 NZ_CP054282:c2768395-2768084 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T159326 NZ_CP070007:4703531-4703638 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT159326 WP_000356397.1 NZ_CP054282:c2768083-2767793 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT159326 NZ_CP070007:c4703478-4703417 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References