Detailed information of TA system TA159281

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2459820..2460550
• Replicon: chromosome
• Accession: NZ_CP054277
• Organism: Serratia marcescens strain Byron

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B4GR63
• Locus tag: HS042_RS11660
• Protein ID: WP_004937033.1
• Coordinates: 2459820..2460131 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HS042_RS11665
• Protein ID: WP_015379178.1
• Coordinates: 2460128..2460550 (+)
• Length: 141 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HS042_RS11630	2455135..2455860	+	726	WP_004937040.1	LPS export ABC transporter ATP-binding protein	-
HS042_RS11635	2455913..2457346	+	1434	WP_021505109.1	RNA polymerase factor sigma-54	-
HS042_RS11640	2457370..2457657	+	288	WP_004937038.1	ribosome hibernation promoting factor	-
HS042_RS11645	2457813..2458274	+	462	WP_016930212.1	PTS IIA-like nitrogen regulatory protein PtsN	-
HS042_RS11650	2458402..2459256	+	855	WP_015379177.1	RNase adapter RapZ	-
HS042_RS11655	2459253..2459525	+	273	WP_004937034.1	PTS phosphocarrier protein NPr	-
HS042_RS11660	2459820..2460131	+	312	WP_004937033.1	type II toxin-antitoxin system HigB family toxin	Toxin
HS042_RS11665	2460128..2460550	+	423	WP_015379178.1	helix-turn-helix domain-containing protein	Antitoxin
HS042_RS11670	2460554..2462470	-	1917	WP_015379179.1	BglG family transcription antiterminator	-
HS042_RS11675	2462522..2463736	-	1215	WP_015379180.1	lactonase family protein	-
HS042_RS11680	2463823..2464563	-	741	WP_004937022.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (81-135)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12289.14 Da
• Isoelectric Point: 10.0957

>T159281 WP_004937033.1 NZ_CP054277:2459820-2460131 [Serratia marcescens]
MHIISREPFNNATKQYPNEATALEAIYKALRRGEFRTPDELKALFPSLDRMKYKEKWWVIDVGGNHLRILFFASFETQKV
FIKHIVSHAEYDKLMQHYRRSSS

Nucleotide

• Length: 312 bp

>T159281 NZ_CP069994:4908667-4908951 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 141 a.a.
• Molecular weight: 15784.13 Da
• Isoelectric Point: 5.2139

>AT159281 WP_015379178.1 NZ_CP054277:2460128-2460550 [Serratia marcescens]
MITDAIKAADALLRAVPLLQGSRAQQDYREALELVEYLLENDEHHPLIDMLAKKIAEYEDNSAEFTAFNQRIAQLPQGVA
ALRVLMEQHQLSQSDFEHEIGKKSLVNQILNGKRSLTIPHIMALAKRFNLPPALFLPEAD

Nucleotide

• Length: 423 bp

>AT159281 NZ_CP069994:4908435-4908677 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A8B4GR63