Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 4690919..4691958 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name PP_4152 Uniprot ID Q88FE5
Locus tag HG554_RS21645 Protein ID WP_010954922.1
Coordinates 4691383..4691958 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A2V4F8J0
Locus tag HG554_RS21640 Protein ID WP_049588231.1
Coordinates 4690919..4691386 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS21625 (HG554_21620) 4687071..4688144 + 1074 WP_010954918.1 microcin C ABC transporter permease YejB -
HG554_RS21630 (HG554_21625) 4688146..4689165 + 1020 WP_010954919.1 ABC transporter permease -
HG554_RS21635 (HG554_21630) 4689167..4690771 + 1605 WP_010954920.1 ABC transporter ATP-binding protein -
HG554_RS21640 (HG554_21635) 4690919..4691386 + 468 WP_049588231.1 helix-turn-helix domain-containing protein Antitoxin
HG554_RS21645 (HG554_21640) 4691383..4691958 + 576 WP_010954922.1 PIN domain-containing protein Toxin
HG554_RS21650 (HG554_21645) 4692172..4695102 + 2931 WP_049588229.1 aminotransferase -
HG554_RS21655 (HG554_21650) 4695106..4696329 - 1224 WP_010954925.1 NAD(P)/FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 4671275..4695102 23827


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21646.86 Da        Isoelectric Point: 5.5710

>T159237 WP_010954922.1 NZ_CP054264:4691383-4691958 [Pseudomonas putida]
MKHSPFTAIYDANVLYPAPLRDFLMNLALTGIYRARWSASIHDEWKRNLLLNRPDLTPEHLDRTSSLMDAAVPDALVTDY
DSLVEGLDLPDRDDRHVLAAAIKCNASVIVTFNLKDFSKAVLGAFDIEPLHPDDFIADLWDLDKAAVLEAAQRQRISLKN
PPHSVQQYLDRLLQQKLPETVKLLSAFKFLL

Download         Length: 576 bp

>T159237 NZ_CP069990:c669716-669342 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17112.54 Da        Isoelectric Point: 6.3403

>AT159237 WP_049588231.1 NZ_CP054264:4690919-4691386 [Pseudomonas putida]
MTTANLSSHVLPDEKEIAVAVESSRTLAAFLSTKSDTQRIELLNEENQRQVVEVPTFALRLFGEILSELALGNSVKVVPI
HAELTTQEAADLLNVSRPHLVKLLDEAVIPHTKTGRHRRVKFSDLVAYKDKRDGVSRAAIDALAAQAQELKMGYE

Download         Length: 468 bp

>AT159237 NZ_CP069990:c670173-669805 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q88FE5

Antitoxin

Source ID Structure
AlphaFold DB A0A2V4F8J0

References