Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1777600..1778186 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name graT Uniprot ID Q88MI5
Locus tag HG554_RS08210 Protein ID WP_010952670.1
Coordinates 1777908..1778186 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID Q88MI6
Locus tag HG554_RS08205 Protein ID WP_010952669.1
Coordinates 1777600..1777899 (-) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS08180 (HG554_08180) 1772860..1774851 + 1992 WP_010952665.1 sialidase family protein -
HG554_RS08185 (HG554_08185) 1774863..1775939 + 1077 WP_010952666.1 hypothetical protein -
HG554_RS08190 (HG554_08190) 1776000..1776443 + 444 WP_049587424.1 structural protein P5 -
HG554_RS08195 (HG554_08195) 1776440..1776940 + 501 WP_010952668.1 DUF2514 domain-containing protein -
HG554_RS08200 (HG554_08200) 1777006..1777308 + 303 WP_049587425.1 hypothetical protein -
HG554_RS08205 (HG554_08205) 1777600..1777899 - 300 WP_010952669.1 type II toxin-antitoxin system antitoxin GraA Antitoxin
HG554_RS08210 (HG554_08210) 1777908..1778186 - 279 WP_010952670.1 type II toxin-antitoxin system toxin GraT Toxin
HG554_RS08215 (HG554_08215) 1778437..1780083 + 1647 WP_049587427.1 Na+/H+ antiporter -
HG554_RS08220 (HG554_08220) 1780189..1781385 - 1197 WP_010952672.1 succinyldiaminopimelate transaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(20-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10446.72 Da        Isoelectric Point: 7.2450

>T159233 WP_010952670.1 NZ_CP054264:c1778186-1777908 [Pseudomonas putida]
MIRSFSCADTEALFTTGKTRRGSDIKSVAERKLAMLDAATELRDLRSPPGNRLESLSGNRADQHSIRVNDQWRLCFTWTE
HGPVNVEIVDYH

Download         Length: 279 bp

>T159233 NZ_CP069982:54244-54549 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10905.55 Da        Isoelectric Point: 5.6793

>AT159233 WP_010952669.1 NZ_CP054264:c1777899-1777600 [Pseudomonas putida]
MLKNGMRPIHPGEILREEFQKEMGFSAAALARALGVATPTVNNILRERGGVSADMALRLSICLDTTPEFWLNLQTAFDLR
TAEQQHGDEIIGSVQRLVA

Download         Length: 300 bp

>AT159233 NZ_CP069982:54024-54242 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6F8S

Antitoxin

Source ID Structure
PDB 6F8S
AlphaFold DB Q88MI6

References