Detailed information of TA system TA159232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 1449221..1450011
• Replicon: chromosome
• Accession: NZ_CP054264
• Organism: Pseudomonas putida strain A0_F0_I1

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: Q88NE5
• Locus tag: HG554_RS06580
• Protein ID: WP_010952397.1
• Coordinates: 1449221..1449688 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: I7C311
• Locus tag: HG554_RS06585
• Protein ID: WP_014859917.1
• Coordinates: 1449685..1450011 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HG554_RS06560 (HG554_06560)	1444384..1445913	+	1530	WP_010952395.1	efflux transporter outer membrane subunit	-
HG554_RS06565 (HG554_06565)	1445910..1448087	+	2178	WP_003251716.1	FUSC family protein	-
HG554_RS06570 (HG554_06570)	1448077..1448277	+	201	WP_003251718.1	DUF1656 domain-containing protein	-
HG554_RS06575 (HG554_06575)	1448288..1449148	+	861	WP_010952396.1	HlyD family secretion protein	-
HG554_RS06580 (HG554_06580)	1449221..1449688	-	468	WP_010952397.1	type II toxin-antitoxin system YhaV family toxin	Toxin
HG554_RS06585 (HG554_06585)	1449685..1450011	-	327	WP_014859917.1	type II toxin-antitoxin system PrlF family antitoxin	Antitoxin
HG554_RS06590 (HG554_06590)	1450104..1450550	-	447	WP_010952399.1	universal stress protein	-
HG554_RS06595 (HG554_06595)	1450824..1451729	+	906	WP_010952400.1	LysR family transcriptional regulator	-
HG554_RS06600 (HG554_06600)	1451846..1453387	+	1542	WP_010952401.1	MFS transporter	-
HG554_RS06605 (HG554_06605)	1453416..1454477	+	1062	WP_049587435.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (17-152)

Antitoxin

• PrlF antitoxin (8-106)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17962.34 Da
• Isoelectric Point: 9.7963

>T159232 WP_010952397.1 NZ_CP054264:c1449688-1449221 [Pseudomonas putida]
MSDASRKPLVIHGWTVIAHPLFLAKLDALSAQVEAQRDKDPTGYTKRNTFKRLAAIRRLAFDVIPQDPTKPEYRQGATLG
GDHKHWFRAKFFQQYRLFFRYHTPSRIIVLAWINDESSKRAYESSDDAYKVFQKMLHSGNPPDDWDQLLQEAAAD

Nucleotide

• Length: 468 bp

>T159232 NZ_CP069982:c49159-48779 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 11919.40 Da
• Isoelectric Point: 4.3722

>AT159232 WP_014859917.1 NZ_CP054264:c1450011-1449685 [Pseudomonas putida]
MAATFDVESTLTDRYQTTVPETVRRALGLKKRDKIHYSIRPDGGVILTRAEATEDDPVLGKFLDFLAHDIATNPQRLQVV
DAGLIARLDKLVGDAEVDLDQPLSADDE

Nucleotide

• Length: 327 bp

>AT159232 NZ_CP069982:c49380-49159 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: Q88NE5

Antitoxin

• AlphaFold DB: I7C311