Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 1373337..1373938 Replicon chromosome
Accession NZ_CP054264
Organism Pseudomonas putida strain A0_F0_I1

Toxin (Protein)


Gene name higB Uniprot ID A0A2L1IAQ2
Locus tag HG554_RS06220 Protein ID WP_049587984.1
Coordinates 1373624..1373938 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2L1IAR7
Locus tag HG554_RS06215 Protein ID WP_049587986.1
Coordinates 1373337..1373624 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HG554_RS06195 (HG554_06195) 1368369..1369214 + 846 WP_003254799.1 DUF6502 family protein -
HG554_RS06200 (HG554_06200) 1369211..1370929 + 1719 WP_010952348.1 DUF5666 domain-containing protein -
HG554_RS06205 (HG554_06205) 1370984..1371733 + 750 WP_049587988.1 hypothetical protein -
HG554_RS06210 (HG554_06210) 1371805..1373136 - 1332 WP_004576169.1 30S ribosomal protein S12 methylthiotransferase RimO -
HG554_RS06215 (HG554_06215) 1373337..1373624 - 288 WP_049587986.1 helix-turn-helix domain-containing protein Antitoxin
HG554_RS06220 (HG554_06220) 1373624..1373938 - 315 WP_049587984.1 hypothetical protein Toxin
HG554_RS06225 (HG554_06225) 1374387..1376297 + 1911 WP_010952353.1 potassium transporter Kup -
HG554_RS06230 (HG554_06230) 1376346..1377638 - 1293 WP_010952354.1 AcvB/VirJ family lysyl-phosphatidylglycerol hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-88)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11784.61 Da        Isoelectric Point: 9.9097

>T159231 WP_049587984.1 NZ_CP054264:c1373938-1373624 [Pseudomonas putida]
MIFIETPVFTSDLKEHLDDEEYRALQAYLAEHPEAGSLLEETGGLRKIRWAAKGKGKSGGVRVIYYHVTAAHQIRMILIY
RKGIVDTLTSSQKAQLRALNKGWK

Download         Length: 315 bp

>T159231 NZ_CP069982:35000-35275 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10841.31 Da        Isoelectric Point: 7.4063

>AT159231 WP_049587986.1 NZ_CP054264:c1373624-1373337 [Pseudomonas putida]
MEKHLFDRLVESMTQMDEIDRGERQPSREFHVDALQVKKIRQATGLSQAAFAKRIDVAVGTLRNWEQGRREPEGPARALL
RAIHNDPEHVLAALS

Download         Length: 288 bp

>AT159231 NZ_CP069982:34716-35000 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2L1IAQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A2L1IAR7

References