Detailed information of TA system TA159211

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/Tad-HTH_37
• Location: 4109153..4109750
• Replicon: chromosome
• Accession: NZ_CP054254
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A0B7GEF2
• Locus tag: HL854_RS19995
• Protein ID: WP_012542526.1
• Coordinates: 4109433..4109750 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HL854_RS19990
• Protein ID: WP_012542525.1
• Coordinates: 4109153..4109440 (-)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS19960	4105063..4105311	+	249	WP_008805539.1	DUF1158 domain-containing protein	-
HL854_RS19965	4105328..4105669	-	342	WP_002893035.1	RamA family antibiotic efflux transcriptional regulator	-
HL854_RS19970	4105700..4106815	-	1116	WP_162493283.1	MBL fold metallo-hydrolase	-
HL854_RS19975	4106995..4107576	+	582	WP_012968754.1	TetR/AcrR family transcriptional regulator	-
HL854_RS19980	4107576..4107944	+	369	WP_008805536.1	MmcQ/YjbR family DNA-binding protein	-
HL854_RS19985	4108064..4108717	+	654	WP_012968755.1	oxygen-insensitive NAD(P)H nitroreductase	-
HL854_RS19990	4109153..4109440	-	288	WP_012542525.1	helix-turn-helix transcriptional regulator	Antitoxin
HL854_RS19995	4109433..4109750	-	318	WP_012542526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HL854_RS20000	4109935..4110978	-	1044	WP_016160437.1	DUF2157 domain-containing protein	-
HL854_RS20005	4111508..4112374	-	867	WP_008805530.1	helix-turn-helix transcriptional regulator	-
HL854_RS20010	4112483..4113910	+	1428	WP_023297179.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-100)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-89)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 12110.39 Da
• Isoelectric Point: 11.2767

>T159211 WP_012542526.1 NZ_CP054254:c4109750-4109433 [Klebsiella variicola]
MFRLVVHVDVKKELQALPPIVQAKMIRQIDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Nucleotide

• Length: 318 bp

>T159211 NZ_CP069980:1135152-1135526 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGTTAAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGTATTGCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10815.51 Da
• Isoelectric Point: 10.2212

>AT159211 WP_012542525.1 NZ_CP054254:c4109440-4109153 [Klebsiella variicola]
MSKKIIDWDTLKTELLSQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQILLY

Nucleotide

• Length: 288 bp

>AT159211 NZ_CP069980:1134694-1135062 [Escherichia coli]
GTGTCAGACACACTTTCCGGGGCAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCAGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0B7GEF2