Detailed information of TA system TA159199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 353408..354054
• Replicon: chromosome
• Accession: NZ_CP054254
• Organism: Klebsiella variicola strain FH-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HL854_RS01605
• Protein ID: WP_032740280.1
• Coordinates: 353408..353755 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A1F2LZT5
• Locus tag: HL854_RS01610
• Protein ID: WP_008806992.1
• Coordinates: 353755..354054 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HL854_RS01595	349295..350728	+	1434	WP_004202133.1	glycogen synthase GlgA	-
HL854_RS01600	350746..353193	+	2448	WP_173817971.1	glycogen phosphorylase	-
HL854_RS01605	353408..353755	+	348	WP_032740280.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HL854_RS01610	353755..354054	+	300	WP_008806992.1	helix-turn-helix transcriptional regulator	Antitoxin
HL854_RS01615	354117..355625	-	1509	WP_032740278.1	glycerol-3-phosphate dehydrogenase	-
HL854_RS01620	355830..356159	+	330	WP_032733885.1	thiosulfate sulfurtransferase GlpE	-
HL854_RS01625	356210..357040	+	831	WP_032740277.1	rhomboid family intramembrane serine protease GlpG	-
HL854_RS01630	357090..357848	+	759	WP_008806995.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (13-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (31-84)

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 13461.49 Da
• Isoelectric Point: 5.6651

>T159199 WP_032740280.1 NZ_CP054254:353408-353755 [Klebsiella variicola]
MWDVETTDTFDAWFELQSRALKEDMLATMLILSEFGPQLGLPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCAGNKDGMNEKRFYKEMITLADREFSQHLTKER

Nucleotide

• Length: 348 bp

>T159199 NZ_CP069978:c3078991-3078614 [Escherichia coli]
ATGAACACATTACCCGATACACACATACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10814.61 Da
• Isoelectric Point: 10.1464

>AT159199 WP_008806992.1 NZ_CP054254:353755-354054 [Klebsiella variicola]
MATLKELMAKQSPESQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Nucleotide

• Length: 300 bp

>AT159199 NZ_CP069978:c3079449-3079081 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A1F2LZT5