Detailed information of TA system TA159191

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 82814..83143
• Replicon: plasmid p1
• Accession: NZ_CP054237
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HR070_RS25035
• Protein ID: WP_001312861.1
• Coordinates: 82814..82972 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83016..83143 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS24995	77926..78153	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
HR070_RS25000	78241..78918	-	678	WP_001348626.1	PAS domain-containing protein	-
HR070_RS25005	79052..79435	-	384	WP_001151566.1	relaxosome protein TraM	-
HR070_RS25010	79766..80368	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HR070_RS25015	80665..81486	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HR070_RS25020	81605..81892	-	288	WP_000107535.1	hypothetical protein	-
HR070_RS25025	81917..82123	-	207	WP_000275859.1	hypothetical protein	-
HR070_RS25030	82036..82371	-	336	WP_013023876.1	hypothetical protein	-
HR070_RS25035	82814..82972	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	83016..83143	-	128	NuclAT_0	-	Antitoxin
-	83016..83143	-	128	NuclAT_0	-	Antitoxin
-	83016..83143	-	128	NuclAT_0	-	Antitoxin
-	83016..83143	-	128	NuclAT_0	-	Antitoxin
-	84585..84687	-	103	NuclAT_1	-	-
-	84585..84687	-	103	NuclAT_1	-	-
-	84585..84687	-	103	NuclAT_1	-	-
-	84585..84687	-	103	NuclAT_1	-	-
HR070_RS25045	84699..85418	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HR070_RS25050	85415..85849	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
HR070_RS25055	85904..87862	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1	-	1..131660	131660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T159191 WP_001312861.1 NZ_CP054237:c82972-82814 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T159191 NZ_CP069978:1280285-1281607 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCACCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGAGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAATGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCAAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACAGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTATGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGTCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Nucleotide

• Length: 128 bp

>AT159191 NZ_CP054237:c83143-83016 [Escherichia coli]
CGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGAAGATAGCCCCGTAGTAA
GTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin