Detailed information of TA system TA159187

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 58547..58786
• Replicon: plasmid p1
• Accession: NZ_CP054237
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HR070_RS24905
• Protein ID: WP_023144756.1
• Coordinates: 58547..58681 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	58726..58786 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS24875	54249..55106	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
HR070_RS24880	55099..55173	-	75	WP_031943482.1	RepA leader peptide Tap	-
HR070_RS24885	55410..55562	-	153	Protein_72	replication protein A	-
HR070_RS24890	55756..57297	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
HR070_RS24895	57312..58058	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
HR070_RS24900	58128..58250	-	123	Protein_75	hypothetical protein	-
HR070_RS24905	58547..58681	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	58726..58786	+	61	NuclAT_2	-	Antitoxin
-	58726..58786	+	61	NuclAT_2	-	Antitoxin
-	58726..58786	+	61	NuclAT_2	-	Antitoxin
-	58726..58786	+	61	NuclAT_2	-	Antitoxin
HR070_RS24910	58753..59039	-	287	Protein_77	DUF2726 domain-containing protein	-
HR070_RS24915	59552..59764	-	213	WP_013023861.1	hypothetical protein	-
HR070_RS24920	59895..60455	-	561	WP_000139341.1	fertility inhibition protein FinO	-
HR070_RS24925	60510..61256	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B / sul2 / aph(3'')-Ib / aph(6)-Id / dfrA1 / ant(3'')-Ia / qacE / sul1	-	1..131660	131660

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T159187 WP_023144756.1 NZ_CP054237:c58681-58547 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T159187 NZ_CP069978:716695-716946 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT159187 NZ_CP054237:58726-58786 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin