Detailed information of TA system TA159185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4930506..4931108
• Replicon: chromosome
• Accession: NZ_CP054236
• Organism: Escherichia coli strain EcPF5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HR070_RS23495
• Protein ID: WP_000897302.1
• Coordinates: 4930797..4931108 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HR070_RS23490
• Protein ID: WP_000356397.1
• Coordinates: 4930506..4930796 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR070_RS23465	4926579..4927481	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HR070_RS23470	4927478..4928113	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HR070_RS23475	4928110..4929039	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HR070_RS23480	4929255..4929473	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HR070_RS23485	4929869..4930147	-	279	WP_001296612.1	hypothetical protein	-
HR070_RS23490	4930506..4930796	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HR070_RS23495	4930797..4931108	-	312	WP_000897302.1	hypothetical protein	Toxin
HR070_RS23500	4931338..4932246	+	909	WP_001305059.1	alpha/beta hydrolase	-
HR070_RS23505	4932310..4933251	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HR070_RS23510	4933296..4933733	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HR070_RS23515	4933730..4934602	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HR070_RS23520	4934596..4935195	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T159185 WP_000897302.1 NZ_CP054236:c4931108-4930797 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159185 NZ_CP069974:c121052-120747 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159185 WP_000356397.1 NZ_CP054236:c4930796-4930506 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159185 NZ_CP069974:c121272-121054 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATACAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: S1P416