Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1488769..1489496 Replicon chromosome
Accession NZ_CP054236
Organism Escherichia coli strain EcPF5

Toxin (Protein)


Gene name higB Uniprot ID V0YLE2
Locus tag HR070_RS07195 Protein ID WP_000547555.1
Coordinates 1488769..1489080 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A0H2V9Z2
Locus tag HR070_RS07200 Protein ID WP_000126293.1
Coordinates 1489077..1489496 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR070_RS07165 1483910..1485619 + 1710 WP_001288134.1 formate hydrogenlyase subunit HycE -
HR070_RS07170 1485629..1486171 + 543 WP_000493797.1 formate hydrogenlyase subunit HycF -
HR070_RS07175 1486171..1486938 + 768 WP_000067399.1 formate hydrogenlyase subunit HycG -
HR070_RS07180 1486935..1487345 + 411 WP_001291918.1 formate hydrogenlyase assembly protein HycH -
HR070_RS07185 1487338..1487808 + 471 WP_000132956.1 hydrogenase maturation peptidase HycI -
HR070_RS07190 1487851..1488606 + 756 WP_001305268.1 hypothetical protein -
HR070_RS07195 1488769..1489080 + 312 WP_000547555.1 type II toxin-antitoxin system HigB family toxin Toxin
HR070_RS07200 1489077..1489496 + 420 WP_000126293.1 helix-turn-helix domain-containing protein Antitoxin
HR070_RS07205 1489588..1490016 - 429 WP_000536064.1 hypothetical protein -
HR070_RS07210 1490401..1490928 + 528 WP_001078777.1 electron transport protein HydN -
HR070_RS07215 1491081..1493333 + 2253 WP_001107659.1 carbamoyltransferase HypF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(22-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(85-138)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12439.18 Da        Isoelectric Point: 9.5146

>T159166 WP_000547555.1 NZ_CP054236:1488769-1489080 [Escherichia coli]
MHIISKAPFEECARKYPNDALALHSLYRVIKETDFSTPEEMRTAFPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Download         Length: 312 bp

>T159166 NZ_CP069973:1829856-1830206 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15426.46 Da        Isoelectric Point: 4.4596

>AT159166 WP_000126293.1 NZ_CP054236:1489077-1489496 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTIPHIKALSARFGVKPEWFL

Download         Length: 420 bp

>AT159166 NZ_CP069973:1829611-1829862 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0YLE2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H2V9Z2

References