Detailed information of TA system TA159144

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-rdlD/SymE(toxin)
• Location: 3538018..3538430
• Replicon: chromosome
• Accession: NZ_CP054232
• Organism: Escherichia coli strain EcPF7

Toxin (Protein)

• Gene name: symE
• Uniprot ID: A0A8E0IWC4
• Locus tag: HR071_RS17015
• Protein ID: WP_001513534.1
• Coordinates: 3538018..3538359 (-)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	rdlD
Locus tag	-
Coordinates	3538354..3538430 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR071_RS16995	3533087..3534367	-	1281	WP_001338077.1	DUF445 domain-containing protein	-
HR071_RS17000	3534552..3535472	+	921	WP_001513532.1	Rpn family recombination-promoting nuclease/putative transposase	-
HR071_RS17005	3535700..3535780	+	81	WP_020233658.1	hypothetical protein	-
HR071_RS17010	3535815..3537971	+	2157	WP_001513533.1	DUF262 domain-containing protein	-
HR071_RS17015	3538018..3538359	-	342	WP_001513534.1	endoribonuclease SymE	Toxin
-	3538354..3538430	+	77	NuclAT_12	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_12	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_12	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_12	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_13	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_13	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_13	-	Antitoxin
-	3538354..3538430	+	77	NuclAT_13	-	Antitoxin
HR071_RS17020	3538580..3540349	-	1770	WP_001513535.1	restriction endonuclease subunit S	-
HR071_RS17025	3540349..3541818	-	1470	WP_001387312.1	type I restriction-modification system subunit M	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12247.03 Da
• Isoelectric Point: 7.8545

>T159144 WP_001513534.1 NZ_CP054232:c3538359-3538018 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASHYPDYSRIPAITLKGQWLEAAGFATGTAVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T159144 NZ_CP069968:c3594100-3593183 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCATCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Nucleotide

• Length: 77 bp

>AT159144 NZ_CP054232:3538354-3538430 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Antitoxin