Detailed information of TA system TA159140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 2903995..2904597
• Replicon: chromosome
• Accession: NZ_CP054232
• Organism: Escherichia coli strain EcPF7

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HR071_RS14020
• Protein ID: WP_000897302.1
• Coordinates: 2903995..2904306 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HR071_RS14025
• Protein ID: WP_000356397.1
• Coordinates: 2904307..2904597 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR071_RS13995	2899909..2900508	+	600	WP_001296610.1	glucose-1-phosphatase	-
HR071_RS14000	2900502..2901374	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HR071_RS14005	2901371..2901808	+	438	WP_000560981.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HR071_RS14010	2901853..2902794	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HR071_RS14015	2902858..2903766	-	909	WP_001385591.1	alpha/beta hydrolase	-
HR071_RS14020	2903995..2904306	+	312	WP_000897302.1	hypothetical protein	Toxin
HR071_RS14025	2904307..2904597	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HR071_RS14030	2904956..2905234	+	279	WP_001296612.1	hypothetical protein	-
HR071_RS14035	2905631..2905849	+	219	WP_001251293.1	ribbon-helix-helix domain-containing protein	-
HR071_RS14040	2906034..2906483	-	450	WP_032140890.1	hypothetical protein	-
HR071_RS14045	2906799..2907647	-	849	WP_001038650.1	hypothetical protein	-
HR071_RS14050	2907937..2908179	+	243	WP_001068514.1	ribbon-helix-helix domain-containing protein	-
HR071_RS14055	2908361..2909290	-	930	WP_000027696.1	formate dehydrogenase accessory protein FdhE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T159140 WP_000897302.1 NZ_CP054232:2903995-2904306 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159140 NZ_CP069968:c2608529-2608212 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159140 WP_000356397.1 NZ_CP054232:2904307-2904597 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159140 NZ_CP069968:c2608219-2607932 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Structures

Toxin

• AlphaFold DB: S1P416