Detailed information of TA system TA159119

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4912970..4913572
• Replicon: chromosome
• Accession: NZ_CP054230
• Organism: Escherichia coli strain EcPF14

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1P416
• Locus tag: HR072_RS23485
• Protein ID: WP_000897302.1
• Coordinates: 4913261..4913572 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HR072_RS23480
• Protein ID: WP_000356397.1
• Coordinates: 4912970..4913260 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR072_RS23455	4909043..4909945	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HR072_RS23460	4909942..4910577	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HR072_RS23465	4910574..4911503	+	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
HR072_RS23470	4911719..4911937	-	219	WP_001314326.1	ribbon-helix-helix domain-containing protein	-
HR072_RS23475	4912333..4912611	-	279	WP_001296612.1	hypothetical protein	-
HR072_RS23480	4912970..4913260	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
HR072_RS23485	4913261..4913572	-	312	WP_000897302.1	hypothetical protein	Toxin
HR072_RS23490	4913802..4914710	+	909	WP_001305059.1	alpha/beta hydrolase	-
HR072_RS23495	4914774..4915715	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HR072_RS23500	4915760..4916197	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
HR072_RS23505	4916194..4917066	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HR072_RS23510	4917060..4917659	-	600	WP_001443191.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12203.19 Da
• Isoelectric Point: 9.7791

>T159119 WP_000897302.1 NZ_CP054230:c4913572-4913261 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T159119 NZ_CP069961:3095520-3095930 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT159119 WP_000356397.1 NZ_CP054230:c4913260-4912970 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT159119 NZ_CP069961:3095273-3095539 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: S1P416