Detailed information of TA system TA159113

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): symE-orzP/SymE(toxin)
• Location: 4335617..4336029
• Replicon: chromosome
• Accession: NZ_CP054230
• Organism: Escherichia coli strain EcPF14

Toxin (Protein)

• Gene name: symE
• Uniprot ID: Q8FA88
• Locus tag: HR072_RS20825
• Protein ID: WP_000132630.1
• Coordinates: 4335688..4336029 (+)
• Length: 114 a.a.

Antitoxin (RNA)

Gene name	orzP
Locus tag	-
Coordinates	4335617..4335693 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR072_RS20815	4332229..4333698	+	1470	WP_001315223.1	type I restriction-modification system subunit M	-
HR072_RS20820	4333698..4335467	+	1770	WP_000110076.1	restriction endonuclease subunit S	-
-	4335617..4335693	-	77	NuclAT_7	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_7	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_7	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_7	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_8	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_8	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_8	-	Antitoxin
-	4335617..4335693	-	77	NuclAT_8	-	Antitoxin
HR072_RS20825	4335688..4336029	+	342	WP_000132630.1	endoribonuclease SymE	Toxin
HR072_RS20830	4336076..4338169	-	2094	WP_000648234.1	DUF262 domain-containing protein	-
HR072_RS20835	4338267..4338347	-	81	WP_020233658.1	hypothetical protein	-
HR072_RS20840	4338575..4339495	-	921	WP_000181195.1	Rpn family recombination-promoting nuclease/putative transposase	-
HR072_RS20845	4339680..4340960	+	1281	WP_001304526.1	DUF445 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SymE-like (20-71)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12294.13 Da
• Isoelectric Point: 8.4982

>T159113 WP_000132630.1 NZ_CP054230:4335688-4336029 [Escherichia coli]
MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKGQWLEAAGFATGTVVDVKVMEGCIVLTAQPPAAAESE
LMQSLRQVCKLSARKQRQVQEFIGVIAGKQKVA

Nucleotide

• Length: 342 bp

>T159113 NZ_CP069961:c1644197-1643856 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGCGAAGCTATATCTGTCCCCCGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
AACTGGCAAGAACAGTCCCCATATCTGCGGGCGGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Nucleotide

• Length: 77 bp

>AT159113 NZ_CP054230:c4335693-4335617 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATTAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A0E0Y5T0

Antitoxin