Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2669553..2670115 Replicon chromosome
Accession NZ_CP054230
Organism Escherichia coli strain EcPF14

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HR072_RS12700 Protein ID WP_000605675.1
Coordinates 2669553..2669831 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HR072_RS12705 Protein ID WP_000781370.1
Coordinates 2669831..2670115 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR072_RS12675 2665139..2665570 - 432 WP_000152307.1 peroxiredoxin OsmC -
HR072_RS12680 2665915..2666130 + 216 WP_000495766.1 biofilm-dependent modulation protein -
HR072_RS12685 2666232..2666369 + 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HR072_RS12690 2666526..2668223 + 1698 WP_000433460.1 malate dehydrogenase -
HR072_RS12695 2668357..2669367 + 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HR072_RS12700 2669553..2669831 + 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR072_RS12705 2669831..2670115 + 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HR072_RS12710 2670344..2670997 - 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HR072_RS12715 2670990..2671874 - 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HR072_RS12720 2671887..2674934 - 3048 WP_011076399.1 formate dehydrogenase-N subunit alpha -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T159109 WP_000605675.1 NZ_CP054230:2669553-2669831 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T159109 NZ_CP069958:66041-66193 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT159109 WP_000781370.1 NZ_CP054230:2669831-2670115 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT159109 NZ_CP069958:c65986-65929 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References