Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3936471..3937270 Replicon chromosome
Accession NZ_CP054227
Organism Escherichia coli strain EcPF15

Toxin (Protein)


Gene name yhaV Uniprot ID B7NDB6
Locus tag HR073_RS18500 Protein ID WP_000347269.1
Coordinates 3936806..3937270 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HR073_RS18495 Protein ID WP_001307405.1
Coordinates 3936471..3936806 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR073_RS18480 3932256..3933026 - 771 WP_176323670.1 2-dehydro-3-deoxyglucarate aldolase -
HR073_RS18485 3933042..3934376 - 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
HR073_RS18490 3934751..3936322 + 1572 WP_001273738.1 galactarate dehydratase -
HR073_RS18495 3936471..3936806 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HR073_RS18500 3936806..3937270 + 465 WP_000347269.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HR073_RS18505 3937325..3938134 - 810 WP_000072171.1 aga operon transcriptional regulator AgaR -
HR073_RS18510 3938383..3939663 + 1281 WP_000681955.1 tagatose-bisphosphate aldolase subunit KbaZ -
HR073_RS18515 3939686..3940159 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HR073_RS18520 3940170..3940949 + 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HR073_RS18525 3940939..3941817 + 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HR073_RS18530 3941835..3942269 + 435 WP_000948824.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3927709..3937270 9561


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17806.22 Da        Isoelectric Point: 9.6924

>T159084 WP_000347269.1 NZ_CP054227:3936806-3937270 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTREAEETH

Download         Length: 465 bp

>T159084 NZ_CP069957:608562-608849 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT159084 WP_001307405.1 NZ_CP054227:3936471-3936806 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159084 NZ_CP069957:608323-608562 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829IY86

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References