Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2155876..2156438 Replicon chromosome
Accession NZ_CP054227
Organism Escherichia coli strain EcPF15

Toxin (Protein)


Gene name higB Uniprot ID L4J948
Locus tag HR073_RS10125 Protein ID WP_000605676.1
Coordinates 2156160..2156438 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HR073_RS10120 Protein ID WP_000781370.1
Coordinates 2155876..2156160 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR073_RS10105 2151058..2154105 + 3048 WP_012602782.1 formate dehydrogenase-N subunit alpha -
HR073_RS10110 2154118..2155002 + 885 WP_001240565.1 formate dehydrogenase N subunit beta -
HR073_RS10115 2154995..2155648 + 654 WP_000045646.1 formate dehydrogenase-N subunit gamma -
HR073_RS10120 2155876..2156160 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HR073_RS10125 2156160..2156438 - 279 WP_000605676.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR073_RS10130 2156624..2157634 - 1011 WP_000642407.1 alcohol dehydrogenase AdhP -
HR073_RS10135 2157768..2159465 - 1698 WP_000433464.1 malate dehydrogenase -
HR073_RS10140 2159622..2159759 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HR073_RS10145 2159861..2160076 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HR073_RS10150 2160421..2160852 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10521.10 Da        Isoelectric Point: 7.3562

>T159071 WP_000605676.1 NZ_CP054227:c2156438-2156160 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAASCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T159071 NZ_CP069946:4171667-4172044 [Klebsiella pneumoniae]
ATGCAAACCCAACCGCTATCCTCCACGCAGGAGGCTTCGCCACGCCCGTCACCGGTGGATATCTGGCAACAGCTTCTGAG
TTACCTGCTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCATTAGGTCACGATGGCGTAATTCAGGAGCATATCG
ACGCGGGTATCTCACTGTGTGACGCCGTAAATTTTATCGTGGAAAAGTACGATCTGGTGCGTACCGATCGTCCCGGTTTC
AGCGCAGAAACACAGTTGCCGCTTCTCAACAGTATCGATATTCTCCGCGCCCGTAAGGCCTGTGGACTGATGACACGCAA
CGACTACAGAACGGTGACCGACATCACCACCGGTAAATACTGTGGGGTGCAGCCATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT159071 WP_000781370.1 NZ_CP054227:c2156160-2155876 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT159071 NZ_CP069946:4171202-4171615 [Klebsiella pneumoniae]
ATGTCGTCCTGCGCAGTGTCACGCTCAATTAACGTTAATTTAAGGAAAGCTAAATTGTCGAATGAAATACCGGCAGTAAA
CCACGATATCGCTGAACCCTGGTGGGGCCTTAAGCCCACCGTCACACCGTGCTTTGGCGCACGACTGGTGCAGGAGGGCA
ACCAGGTGCACTATCTCGCCGACCGCGCCAGCATCACCGGTCAGTTCAGCGAGGCGGATTTACGCCATCTTGACCAAGCC
TTTCCGGTACTACTCAAACAGCTGGAATTGATGCTGGTTTCCGGTGAACTCAATCCTCACCATCAGCACGGCGTTACGCT
CTACGCAAAAGGGTTGACCTGCGAAGCCGATTCACTCGGCTCGCATGGCTACATTTATATTGCGATTTATCCAACGCCTG
CAGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829G6T1

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References