Detailed information of TA system TA159057

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 101620..102347
• Replicon: plasmid p1
• Accession: NZ_CP054225
• Organism: Escherichia coli strain EcPF16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: HR074_RS23440
• Protein ID: WP_176321126.1
• Coordinates: 102036..102347 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: HR074_RS23435
• Protein ID: WP_000990392.1
• Coordinates: 101620..102039 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR074_RS23410	96757..97496	+	740	Protein_103	IS3 family transposase	-
HR074_RS23415	98169..99677	+	1509	WP_001189111.1	group II intron reverse transcriptase/maturase	-
HR074_RS23420	99767..100240	+	474	Protein_105	IS3 family transposase	-
HR074_RS23425	100285..100761	-	477	Protein_106	recombinase family protein	-
HR074_RS23430	100814..101518	-	705	WP_009364894.1	IS6-like element IS26 family transposase	-
HR074_RS23435	101620..102039	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
HR074_RS23440	102036..102347	-	312	WP_176321126.1	type II toxin-antitoxin system HigB family toxin	Toxin
HR074_RS23445	102577..105468	-	2892	Protein_110	Tn3 family transposase	-
HR074_RS23450	105522..106226	-	705	WP_009364894.1	IS6-like element IS26 family transposase	-
HR074_RS23455	106217..106405	+	189	WP_176321123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..123935	123935
	-	inside	IScluster/Tn	-	-	76813..105516	28703

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12344.10 Da
• Isoelectric Point: 10.0671

>T159057 WP_176321126.1 NZ_CP054225:c102347-102036 [Escherichia coli]
MHVVSRAPFDTATRQFPNQAAALDDVYRTLKKENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T159057 NZ_CP069945:c3901468-3901061 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT159057 WP_000990392.1 NZ_CP054225:c102039-101620 [Escherichia coli]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT159057 NZ_CP069945:c3901715-3901449 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: V0AHC4