Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1849406..1849908 Replicon chromosome
Accession NZ_CP054224
Organism Escherichia coli strain EcPF16

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag HR074_RS08795 Protein ID WP_000767829.1
Coordinates 1849654..1849908 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag HR074_RS08790 Protein ID WP_001259255.1
Coordinates 1849406..1849657 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR074_RS08765 1844584..1845651 - 1068 WP_032238524.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
HR074_RS08770 1845651..1846721 - 1071 WP_000108963.1 histidinol-phosphate transaminase -
HR074_RS08775 1846718..1848022 - 1305 WP_176321020.1 histidinol dehydrogenase -
HR074_RS08780 1848028..1848927 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
HR074_RS08785 1849073..1849123 - 51 WP_001364200.1 his operon leader peptide -
HR074_RS08790 1849406..1849657 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HR074_RS08795 1849654..1849908 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HR074_RS08800 1849991..1850815 + 825 WP_000754752.1 SDR family oxidoreductase -
HR074_RS08805 1850861..1851790 + 930 WP_032177447.1 LysR family transcriptional regulator -
HR074_RS08810 1852005..1852067 + 63 WP_010723108.1 membrane protein YoeI -
HR074_RS08815 1852057..1853415 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
HR074_RS08820 1853594..1854652 + 1059 WP_000492346.1 transport protein YeeE -
HR074_RS08825 1854666..1854893 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T159040 WP_000767829.1 NZ_CP054224:1849654-1849908 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T159040 NZ_CP069945:443194-443367 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT159040 WP_001259255.1 NZ_CP054224:1849406-1849657 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT159040 NZ_CP069945:442870-443166 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTTTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References