Detailed information of TA system TA159036

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 686273..687000
• Replicon: chromosome
• Accession: NZ_CP054224
• Organism: Escherichia coli strain EcPF16

Toxin (Protein)

• Gene name: higB
• Uniprot ID: S1EX98
• Locus tag: HR074_RS03400
• Protein ID: WP_000550192.1
• Coordinates: 686273..686587 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HR074_RS03405
• Protein ID: WP_000560266.1
• Coordinates: 686584..687000 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR074_RS03380	682440..683426	-	987	WP_032238269.1	Gfo/Idh/MocA family oxidoreductase	-
HR074_RS03385	683505..684188	-	684	WP_001183046.1	vancomycin high temperature exclusion protein	-
HR074_RS03390	684265..684768	-	504	WP_001295542.1	M48 family metallopeptidase	-
HR074_RS03395	684853..685989	+	1137	WP_000018695.1	23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG	-
HR074_RS03400	686273..686587	+	315	WP_000550192.1	type II toxin-antitoxin system toxin HigB	Toxin
HR074_RS03405	686584..687000	+	417	WP_000560266.1	type II toxin-antitoxin system antitoxin HigA	Antitoxin
HR074_RS03410	687045..689063	-	2019	WP_000121433.1	NADPH-dependent 2,4-dienoyl-CoA reductase	-
HR074_RS03415	689489..691840	-	2352	WP_000695486.1	alpha-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-135)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12063.08 Da
• Isoelectric Point: 10.0409

>T159036 WP_000550192.1 NZ_CP054224:686273-686587 [Escherichia coli]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTPKEYDFFTAVHRTKGKK

Nucleotide

• Length: 315 bp

>T159036 NZ_CP069943:5399142-5399360 [Klebsiella oxytoca]
ATGTCTGATAAAACATTAACCAAAATAGACTATTTGATGCGCTTACGACGTTGTCAGACAATTGATACGCTGGAGCGCGT
AATTGAAAAAAATAAATATGAACTTTCTGATAATGAACTGGCGGTATTTTACTCAGCAGCCGATCATCGTCTTGCTGAAC
TGACGATGAATAAACTGTATGACAAGATCCCGCCATCGGTCTGGAAATTCATCCGCTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 14995.42 Da
• Isoelectric Point: 4.4547

>AT159036 WP_000560266.1 NZ_CP054224:686584-687000 [Escherichia coli]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Nucleotide

• Length: 417 bp

>AT159036 NZ_CP069943:5398741-5399115 [Klebsiella oxytoca]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCGCAGCTTAAATTTCTCTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTTAATGACCCAACCTCAGCTGTCAATCTTCAGCTTAATGAATTGATCGAGC
ACATTGCCGCGTTCGCACTTAATTATAAAATTAAGTATGCTGAGGATAATAAGCTGGTGACTCAGCTTGACGAATATCTG
GATGACACTTTCGTGTTGTTCAGCAACTACGGCATTAACACTGCAGATCTGCAGAAATGGCGTAAATCAGGCAACAGGTT
ATTCCGCTGCTTTGTGAACGCCAGTCGGGAAAACCCGGCCAGTCTTTCTTGTTAA

Structures

Toxin

• AlphaFold DB: S1EX98