Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 635399..636198 Replicon chromosome
Accession NZ_CP054224
Organism Escherichia coli strain EcPF16

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag HR074_RS03140 Protein ID WP_000347251.1
Coordinates 635399..635863 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HR074_RS03145 Protein ID WP_001307405.1
Coordinates 635863..636198 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR074_RS03110 630400..630834 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
HR074_RS03115 630852..631730 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
HR074_RS03120 631720..632499 - 780 WP_000406214.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
HR074_RS03125 632510..632983 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HR074_RS03130 633006..634286 - 1281 WP_000681905.1 tagatose-bisphosphate aldolase subunit KbaZ -
HR074_RS03135 634535..635344 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HR074_RS03140 635399..635863 - 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HR074_RS03145 635863..636198 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HR074_RS03150 636347..637918 - 1572 WP_001273753.1 galactarate dehydratase -
HR074_RS03155 638293..639627 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HR074_RS03160 639643..640413 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T159035 WP_000347251.1 NZ_CP054224:c635863-635399 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T159035 NZ_CP069943:3256298-3256401 [Klebsiella oxytoca]
GGCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTTTTTTT

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT159035 WP_001307405.1 NZ_CP054224:c636198-635863 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT159035 NZ_CP069943:c3256408-3256263 [Klebsiella oxytoca]
AAAAGATAAAAAAAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCCTTATAAGAATAGTTTACCGTGTCAGGTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References