Detailed information of TA system TA159030

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 40266..40530
• Replicon: plasmid p2
• Accession: NZ_CP054221
• Organism: Escherichia coli strain EcPF18

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: HR075_RS25205
• Protein ID: WP_001331364.1
• Coordinates: 40378..40530 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	40266..40323 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR075_RS25190	35521..37812	-	2292	WP_001289276.1	hypothetical protein	-
HR075_RS25195	37805..38875	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
HR075_RS25200	38894..40102	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	40266..40323	-	58	NuclAT_0	-	Antitoxin
-	40266..40323	-	58	NuclAT_0	-	Antitoxin
-	40266..40323	-	58	NuclAT_0	-	Antitoxin
-	40266..40323	-	58	NuclAT_0	-	Antitoxin
HR075_RS25205	40378..40530	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
HR075_RS25210	40602..40853	-	252	WP_176305162.1	hypothetical protein	-
HR075_RS25215	41512..41688	-	177	WP_001054900.1	hypothetical protein	-
HR075_RS25220	42080..42289	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
HR075_RS25225	42361..43011	-	651	WP_001178507.1	plasmid IncI1-type surface exclusion protein ExcA	-
HR075_RS25230	43085..45253	-	2169	WP_000698358.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..86740	86740

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T159030 WP_001331364.1 NZ_CP054221:40378-40530 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T159030 NZ_CP069941:c141731-141261 [Klebsiella pneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
TTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGGCAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCACTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Nucleotide

• Length: 58 bp

>AT159030 NZ_CP054221:c40323-40266 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin