Detailed information of TA system TA159024

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 88587..88829
• Replicon: plasmid p1
• Accession: NZ_CP054220
• Organism: Escherichia coli strain EcPF18

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HR075_RS24735
• Protein ID: WP_001312861.1
• Coordinates: 88587..88745 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	88789..88829 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR075_RS24695	83699..83926	-	228	WP_001254388.1	conjugal transfer relaxosome protein TraY	-
HR075_RS24700	84014..84691	-	678	WP_001348626.1	PAS domain-containing protein	-
HR075_RS24705	84825..85208	-	384	WP_001151566.1	relaxosome protein TraM	-
HR075_RS24710	85539..86141	+	603	WP_000243712.1	transglycosylase SLT domain-containing protein	-
HR075_RS24715	86438..87259	-	822	WP_001234469.1	DUF945 domain-containing protein	-
HR075_RS24720	87378..87665	-	288	WP_000107535.1	hypothetical protein	-
HR075_RS24725	87690..87896	-	207	WP_000275859.1	hypothetical protein	-
HR075_RS24730	87809..88144	-	336	WP_013023876.1	hypothetical protein	-
HR075_RS24735	88587..88745	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	88789..88829	-	41	NuclAT_1	-	Antitoxin
-	88789..88829	-	41	NuclAT_1	-	Antitoxin
-	88789..88829	-	41	NuclAT_1	-	Antitoxin
-	88789..88829	-	41	NuclAT_1	-	Antitoxin
-	90273..90459	-	187	NuclAT_0	-	-
-	90273..90459	-	187	NuclAT_0	-	-
-	90273..90459	-	187	NuclAT_0	-	-
-	90273..90459	-	187	NuclAT_0	-	-
HR075_RS24745	90471..91190	-	720	WP_001276232.1	plasmid SOS inhibition protein A	-
HR075_RS24750	91187..91621	-	435	WP_000845940.1	conjugation system SOS inhibitor PsiB	-
HR075_RS24755	91676..93634	-	1959	WP_000117179.1	ParB/RepB/Spo0J family partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..128954	128954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T159024 WP_001312861.1 NZ_CP054220:c88745-88587 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T159024 NZ_CP069940:2558344-2558727 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTTGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGGTTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 41 bp

>AT159024 NZ_CP054220:c88829-88789 [Escherichia coli]
TTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin