Detailed information of TA system TA159020

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 47827..48066
• Replicon: plasmid p1
• Accession: NZ_CP054220
• Organism: Escherichia coli strain EcPF18

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HR075_RS24500
• Protein ID: WP_023144756.1
• Coordinates: 47827..47961 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	48006..48066 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR075_RS24470	43838..44239	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HR075_RS24475	44172..44429	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HR075_RS24480	44522..45175	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HR075_RS24485	46115..46972	-	858	WP_001617855.1	incFII family plasmid replication initiator RepA	-
HR075_RS24490	46965..47039	-	75	WP_031943482.1	RepA leader peptide Tap	-
HR075_RS24495	47276..47530	-	255	WP_000083850.1	replication regulatory protein RepA	-
HR075_RS24500	47827..47961	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	48006..48066	+	61	NuclAT_2	-	Antitoxin
-	48006..48066	+	61	NuclAT_2	-	Antitoxin
-	48006..48066	+	61	NuclAT_2	-	Antitoxin
-	48006..48066	+	61	NuclAT_2	-	Antitoxin
HR075_RS24505	48033..48319	-	287	Protein_54	DUF2726 domain-containing protein	-
HR075_RS24510	48397..50010	-	1614	WP_000080195.1	IS66-like element ISEc23 family transposase	-
HR075_RS24515	50041..50391	-	351	WP_000624722.1	IS66 family insertion sequence element accessory protein TnpB	-
HR075_RS24520	50388..50813	-	426	WP_000422741.1	IS66 family insertion sequence hypothetical protein	-
HR075_RS24525	51372..51584	-	213	WP_013023861.1	hypothetical protein	-
HR075_RS24530	51715..52275	-	561	WP_000139341.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1B	senB	1..128954	128954

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T159020 WP_023144756.1 NZ_CP054220:c47961-47827 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T159020 NZ_CP069940:c456347-456245 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 61 bp

>AT159020 NZ_CP054220:48006-48066 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin