159015

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 4083188..4083867 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag HR075_RS19425 Protein ID WP_000057523.1
Coordinates 4083188..4083490 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag HR075_RS19430 Protein ID WP_000806442.1
Coordinates 4083526..4083867 (+) Length 114 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS19400 4078564..4080216 + 1653 WP_000771748.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase -
HR075_RS19405 4080254..4080757 - 504 WP_000667000.1 hypothetical protein -
HR075_RS19410 4080754..4081554 - 801 WP_000439798.1 hypothetical protein -
HR075_RS19415 4081578..4082057 - 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
HR075_RS19420 4082261..4083055 - 795 WP_000365147.1 TraB/GumN family protein -
HR075_RS19425 4083188..4083490 + 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR075_RS19430 4083526..4083867 + 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
HR075_RS19435 4083925..4086429 - 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
HR075_RS19440 4086691..4087623 + 933 WP_000883041.1 glutaminase A -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(9-98)

Antitoxin

(1-100)


Sequences


Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T159015 WP_000057523.1 NZ_CP054219:4083188-4083490 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T159015 NZ_CP069939:3188474-3188842 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT159015 WP_000806442.1 NZ_CP054219:4083526-4083867 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT159015 NZ_CP069939:3188256-3188477 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB S1PK60


Antitoxin

Source ID Structure
AlphaFold DB S1QAY3

References