Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 2890538..2891140 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name higB Uniprot ID S1P416
Locus tag HR075_RS13820 Protein ID WP_000897302.1
Coordinates 2890538..2890849 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag HR075_RS13825 Protein ID WP_000356397.1
Coordinates 2890850..2891140 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS13795 2886452..2887051 + 600 WP_001296610.1 glucose-1-phosphatase -
HR075_RS13800 2887045..2887917 + 873 WP_000920762.1 virulence factor BrkB family protein -
HR075_RS13805 2887914..2888351 + 438 WP_000560981.1 D-tyrosyl-tRNA(Tyr) deacylase -
HR075_RS13810 2888396..2889337 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HR075_RS13815 2889401..2890309 - 909 WP_001385591.1 alpha/beta hydrolase -
HR075_RS13820 2890538..2890849 + 312 WP_000897302.1 hypothetical protein Toxin
HR075_RS13825 2890850..2891140 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
HR075_RS13830 2891499..2891777 + 279 WP_001296612.1 hypothetical protein -
HR075_RS13835 2892174..2892392 + 219 WP_001251293.1 ribbon-helix-helix domain-containing protein -
HR075_RS13840 2892577..2893026 - 450 WP_032140890.1 hypothetical protein -
HR075_RS13845 2893342..2894190 - 849 WP_001038650.1 hypothetical protein -
HR075_RS13850 2894480..2894722 + 243 WP_001068514.1 ribbon-helix-helix domain-containing protein -
HR075_RS13855 2894904..2895833 - 930 WP_000027696.1 formate dehydrogenase accessory protein FdhE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12203.19 Da        Isoelectric Point: 9.7791

>T159006 WP_000897302.1 NZ_CP054219:2890538-2890849 [Escherichia coli]
MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T159006 NZ_CP069935:c4641330-4641178 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT159006 WP_000356397.1 NZ_CP054219:2890850-2891140 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT159006 NZ_CP069935:4641379-4641436 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P416

Antitoxin

Source ID Structure

References