Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 816420..816922 Replicon chromosome
Accession NZ_CP054219
Organism Escherichia coli strain EcPF18

Toxin (Protein)


Gene name yoeB Uniprot ID E2QNP2
Locus tag HR075_RS04120 Protein ID WP_000767819.1
Coordinates 816420..816674 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID E2QNP3
Locus tag HR075_RS04125 Protein ID WP_001259253.1
Coordinates 816671..816922 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR075_RS04095 812238..812696 - 459 WP_001531805.1 IS200/IS605-like element IS200C family transposase -
HR075_RS04100 812913..814271 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
HR075_RS04105 814261..814323 - 63 WP_010723108.1 membrane protein YoeI -
HR075_RS04110 814538..815467 - 930 WP_000803366.1 LysR family transcriptional regulator -
HR075_RS04115 815513..816337 - 825 WP_000754737.1 SDR family oxidoreductase -
HR075_RS04120 816420..816674 - 255 WP_000767819.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
HR075_RS04125 816671..816922 - 252 WP_001259253.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
HR075_RS04130 817205..817255 + 51 WP_001364200.1 his operon leader peptide -
HR075_RS04135 817401..818300 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
HR075_RS04140 818306..819610 + 1305 WP_001296211.1 histidinol dehydrogenase -
HR075_RS04145 819607..820677 + 1071 WP_000108967.1 histidinol-phosphate transaminase -
HR075_RS04150 820677..821744 + 1068 WP_000080057.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 812238..812696 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10110.53 Da        Isoelectric Point: 7.2749

>T159000 WP_000767819.1 NZ_CP054219:c816674-816420 [Escherichia coli]
VKLIWSEESWDDYLYWQEADKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHLLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T159000 NZ_CP069935:c3505845-3505738 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9365.62 Da        Isoelectric Point: 4.6127

>AT159000 WP_001259253.1 NZ_CP054219:c816922-816671 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSDKGTEKD
IIE

Download         Length: 252 bp

>AT159000 NZ_CP069935:3505902-3505959 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB E2QNP2

Antitoxin

Source ID Structure
AlphaFold DB E2QNP3

References