Detailed information of TA system TA158979

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 4047937..4048630
• Replicon: chromosome
• Accession: NZ_CP054214
• Organism: Escherichia coli strain EcPF40

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: HR076_RS19620
• Protein ID: WP_000415584.1
• Coordinates: 4048334..4048630 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: HR076_RS19615
• Protein ID: WP_000650107.1
• Coordinates: 4047937..4048332 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR076_RS19605	4043801..4046059	-	2259	WP_001281841.1	DNA topoisomerase IV subunit A	-
HR076_RS19610	4046197..4047804	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
HR076_RS19615	4047937..4048332	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
HR076_RS19620	4048334..4048630	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
HR076_RS19625	4048835..4049317	-	483	WP_000183505.1	transcriptional regulator	-
HR076_RS19630	4049370..4049762	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
HR076_RS19635	4049914..4050573	+	660	WP_001221493.1	two-component system response regulator QseB	-
HR076_RS19640	4050570..4051919	+	1350	WP_000673402.1	two-component system sensor histidine kinase QseC	-
HR076_RS19645	4051965..4052297	-	333	WP_000917685.1	DUF2645 family protein	-
HR076_RS19650	4052616..4053197	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
HR076_RS19655	4053228..4053542	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4039474..4048630	9156

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T158979 WP_000415584.1 NZ_CP054214:c4048630-4048334 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T158979 NZ_CP069907:4359009-4359293 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT158979 WP_000650107.1 NZ_CP054214:c4048332-4047937 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT158979 NZ_CP069907:4358777-4359019 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• AlphaFold DB: A0A7U9LU65