158967

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 2309105..2309667 Replicon chromosome
Accession NZ_CP054214
Organism Escherichia coli strain EcPF40

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag HR076_RS11305 Protein ID WP_000605675.1
Coordinates 2309389..2309667 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag HR076_RS11300 Protein ID WP_000781370.1
Coordinates 2309105..2309389 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HR076_RS11285 2304286..2307333 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
HR076_RS11290 2307346..2308230 + 885 WP_001240584.1 formate dehydrogenase N subunit beta -
HR076_RS11295 2308223..2308876 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
HR076_RS11300 2309105..2309389 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
HR076_RS11305 2309389..2309667 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HR076_RS11310 2309853..2310863 - 1011 WP_000642412.1 alcohol dehydrogenase AdhP -
HR076_RS11315 2310997..2312694 - 1698 WP_000433460.1 malate dehydrogenase -
HR076_RS11320 2312851..2312988 - 138 WP_000841554.1 stationary-phase-induced ribosome-associated protein -
HR076_RS11325 2313090..2313305 - 216 WP_000495766.1 biofilm-dependent modulation protein -
HR076_RS11330 2313650..2314081 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T158967 WP_000605675.1 NZ_CP054214:c2309667-2309389 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T158967 NZ_CP069904:c332750-332463 [Klebsiella pneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT158967 WP_000781370.1 NZ_CP054214:c2309389-2309105 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT158967 NZ_CP069904:c332989-332750 [Klebsiella pneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CUG6

References