Detailed information of TA system TA158964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 1002900..1003594
• Replicon: chromosome
• Accession: NZ_CP054214
• Organism: Escherichia coli strain EcPF40

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1PJQ2
• Locus tag: HR076_RS04790
• Protein ID: WP_001263500.1
• Coordinates: 1003196..1003594 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: HR076_RS04785
• Protein ID: WP_000554758.1
• Coordinates: 1002900..1003193 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HR076_RS04760	998717..999184	+	468	WP_000725261.1	flagellar basal body-associated FliL family protein	-
HR076_RS04765	999204..999920	+	717	WP_000938731.1	FliA/WhiG family RNA polymerase sigma factor	-
HR076_RS04770	999933..1000796	+	864	WP_001169518.1	flagellar motor stator protein MotA	-
HR076_RS04775	1000799..1001722	+	924	WP_001532973.1	putative lateral flagellar export/assembly protein LafU	-
HR076_RS04780	1001793..1002848	+	1056	WP_001226168.1	DNA polymerase IV	-
HR076_RS04785	1002900..1003193	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
HR076_RS04790	1003196..1003594	+	399	WP_001263500.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
HR076_RS04795	1003604..1004056	+	453	WP_001059895.1	GNAT family N-acetyltransferase	-
HR076_RS04800	1004234..1005385	+	1152	WP_001335538.1	RNA ligase RtcB family protein	-
HR076_RS04805	1005382..1005996	+	615	WP_000602124.1	peptide chain release factor H	-
HR076_RS04810	1006053..1007510	-	1458	WP_001292999.1	cytosol nonspecific dipeptidase	-
HR076_RS04815	1007771..1008229	+	459	WP_001291991.1	xanthine phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15413.85 Da
• Isoelectric Point: 8.9511

>T158964 WP_001263500.1 NZ_CP054214:1003196-1003594 [Escherichia coli]
MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDAAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T158964 NZ_CP069904:c29772-29356 [Klebsiella pneumoniae]
GTGAACAAAACCTATATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCATTAAGCGCCT
GGAGCAGGCGGTGCTGCGCAACCACCGGATTGTGGTTTCGGCCATCACCTATGCCGAAATGCGCTTCGGGGCGATAGGCA
GGAAGGCCCCGCCCCGCCACGCCCAGCTGGTTGACGCCTTCTGCGCCCGCCTCGATTCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGATGCCACCACAGAAATTAAAGCGACACTGGCCGCAGCCGGAACCCCGATCGGCCCAAACGACACGGCCATCGC
CGGGCACGCCATCGCTGCCGGCGCCGTTCTGGTGACCAATAATGTACGGGAGTTTGCACGGGTGCCGGGGCTGGTACTGG
AAGACTGGGCTAGCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT158964 WP_000554758.1 NZ_CP054214:1002900-1003193 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT158964 NZ_CP069904:c29999-29769 [Klebsiella pneumoniae]
ATGAGGACCGTATCCATTTTTAAGAACGGCAACAACCGCGCCATCCGTCTGCCCCGCGATCTGGATTTTGAGGGCGTGAG
CGAGCTGGAGATTGTCCGGGACGGGGACAGCATTGTCCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCAGAGCTGG
AAAAGGCCGACGCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGTGACGAAGGACGCTTTGATCTGTGA

Structures

Toxin

• AlphaFold DB: S1PJQ2

Antitoxin

• AlphaFold DB: S1QAE3