Detailed information of TA system TA158947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 1385642..1386285
• Replicon: chromosome
• Accession: NZ_CP054198
• Organism: Glaesserella parasuis strain YHP170504

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U4SMB1
• Locus tag: HQ939_RS07120
• Protein ID: WP_005714197.1
• Coordinates: 1385941..1386285 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HQ939_RS07115
• Protein ID: WP_012621770.1
• Coordinates: 1385642..1385938 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ939_RS07095	1382039..1382665	-	627	WP_021110879.1	23S rRNA (uridine(2552)-2'-O)-methyltransferase RlmE	-
HQ939_RS07100	1382978..1383766	+	789	WP_021117032.1	SDR family oxidoreductase	-
HQ939_RS07105	1383883..1384305	-	423	WP_043894462.1	helix-turn-helix transcriptional regulator	-
HQ939_RS07110	1384452..1385516	+	1065	WP_075621097.1	DUF3944 domain-containing protein	-
HQ939_RS07115	1385642..1385938	-	297	WP_012621770.1	helix-turn-helix domain-containing protein	Antitoxin
HQ939_RS07120	1385941..1386285	-	345	WP_005714197.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQ939_RS07125	1386500..1388479	+	1980	WP_075606061.1	exoribonuclease II	-
HQ939_RS07130	1388590..1389681	+	1092	WP_005714201.1	murein transglycosylase A	-
HQ939_RS07135	1389864..1390460	-	597	WP_021110885.1	DedA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (52-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-76)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13263.22 Da
• Isoelectric Point: 5.9233

>T158947 WP_005714197.1 NZ_CP054198:c1386285-1385941 [Glaesserella parasuis]
METEEYLTFIETKVFEEDRKALMSDDEYQKFQAYLLESHELGDFIQNTGGCQKIRWKLESNNKGKSGGVRVIYYVVTKQG
KLLLMMMYAKSKQDNMSDKQKAMLKAVVSQLSEE

Nucleotide

• Length: 345 bp

>T158947 NZ_CP069893:2693206-2693517 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11224.13 Da
• Isoelectric Point: 10.0887

>AT158947 WP_012621770.1 NZ_CP054198:c1385938-1385642 [Glaesserella parasuis]
MEKELFERLMESAREMVAIEKGEIEVPPHRIHIHKIPNVKAIRTQHQMKQAEFASLLGVSQNLVQSWEIGRRTPNGAARK
LLAIIEQQPNLVPVLQNL

Nucleotide

• Length: 297 bp

>AT158947 NZ_CP069893:2693518-2693808 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: U4SMB1