Detailed information of TA system TA158936

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 193236..193662
• Replicon: plasmid pHNTH9F11-1
• Accession: NZ_CP054192
• Organism: Escherichia coli strain TH9F11

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HRL54_RS24690
• Protein ID: WP_001372321.1
• Coordinates: 193236..193361 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	193438..193662 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRL54_RS24650 (188605)	188605..189294	-	690	WP_000283385.1	conjugal transfer transcriptional regulator TraJ	-
HRL54_RS24655 (189481)	189481..189864	-	384	WP_001151524.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HRL54_RS24660 (190185)	190185..190787	+	603	WP_000243709.1	transglycosylase SLT domain-containing protein	-
HRL54_RS24665 (191084)	191084..191905	-	822	WP_001234469.1	DUF932 domain-containing protein	-
HRL54_RS24670 (192027)	192027..192314	-	288	WP_000107535.1	hypothetical protein	-
HRL54_RS24675 (192339)	192339..192545	-	207	WP_000547939.1	hypothetical protein	-
HRL54_RS24680 (192615)	192615..192788	+	174	Protein_208	hypothetical protein	-
HRL54_RS24685 (192786)	192786..193016	-	231	WP_071587244.1	hypothetical protein	-
HRL54_RS24690 (193236)	193236..193361	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
HRL54_RS24695 (193303)	193303..193452	-	150	Protein_211	plasmid maintenance protein Mok	-
- (193438)	193438..193662	-	225	NuclAT_0	-	Antitoxin
- (193438)	193438..193662	-	225	NuclAT_0	-	Antitoxin
- (193438)	193438..193662	-	225	NuclAT_0	-	Antitoxin
- (193438)	193438..193662	-	225	NuclAT_0	-	Antitoxin
HRL54_RS24700 (193474)	193474..193662	+	189	WP_001299721.1	hypothetical protein	-
HRL54_RS24705 (193631)	193631..194393	-	763	Protein_213	plasmid SOS inhibition protein A	-
HRL54_RS24710 (194390)	194390..194824	-	435	WP_000845901.1	conjugation system SOS inhibitor PsiB	-
HRL54_RS24715 (194879)	194879..196837	-	1959	WP_124736966.1	ParB/RepB/Spo0J family partition protein	-
HRL54_RS24720 (196896)	196896..197129	-	234	WP_000006012.1	DUF905 family protein	-
HRL54_RS24725 (197185)	197185..197712	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
HRL54_RS24730 (198185)	198185..198445	-	261	WP_072132736.1	hypothetical protein	-
HRL54_RS24735 (198355)	198355..198597	-	243	WP_001610271.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aadA2 / cmlA1 / ant(3'')-Ia / sul3 / aac(3)-IVa / aph(4)-Ia / aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / blaNDM-5 / tet(A) / blaCTX-M-55 / fosA3 / dfrA1 / sitABCD / qnrS1 / ARR-3 / blaOXA-10 / dfrA14 / floR	iroB / iroC / iroD / iroE / iroN	1..407456	407456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T158936 WP_001372321.1 NZ_CP054192:c193361-193236 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T158936 NZ_CP069892:1389212-1389466 [Streptococcus anginosus]
ATGTTTAACTTTACAGAACAAGCTTGGAAAGATTATGTTTCTTGGCAACAAGAGGATAAAAAGATACTCAAACGTATCAA
TCGTTTAATAGAGGATATCAAACGGAATCCTTTTGAAGGGATTGGAAAGCCTGAACCCTTAAAATATCGTTATTCCGGTG
CTTGGTCTCGTAGAATTACAGATGAACAACAATTAGTCTATATGGTGGAAAAAGATGAGATTTATTTCTTATCCTTTAGA
GACCATTACACATAA

Antitoxin

Nucleotide

• Length: 225 bp

>AT158936 NZ_CP054192:c193662-193438 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin