Detailed information of TA system TA158933

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4686549..4687151
• Replicon: chromosome
• Accession: NZ_CP054191
• Organism: Escherichia coli strain TH9F11

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HRL54_RS22680
• Protein ID: WP_000897305.1
• Coordinates: 4686840..4687151 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRL54_RS22675
• Protein ID: WP_000356397.1
• Coordinates: 4686549..4686839 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRL54_RS22650 (4682494)	4682494..4683396	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HRL54_RS22655 (4683393)	4683393..4684028	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HRL54_RS22660 (4684025)	4684025..4684954	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HRL54_RS22665 (4685284)	4685284..4685526	-	243	WP_001087409.1	protein YiiF	-
HRL54_RS22670 (4685745)	4685745..4685963	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HRL54_RS22675 (4686549)	4686549..4686839	-	291	WP_000356397.1	NadS family protein	Antitoxin
HRL54_RS22680 (4686840)	4686840..4687151	-	312	WP_000897305.1	hypothetical protein	Toxin
HRL54_RS22685 (4687380)	4687380..4688288	+	909	WP_001162704.1	alpha/beta hydrolase	-
HRL54_RS22690 (4688352)	4688352..4689293	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HRL54_RS22695 (4689338)	4689338..4689775	-	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HRL54_RS22700 (4689772)	4689772..4690644	-	873	WP_000920762.1	virulence factor BrkB family protein	-
HRL54_RS22705 (4690638)	4690638..4691237	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T158933 WP_000897305.1 NZ_CP054191:c4687151-4686840 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T158933 NZ_CP069890:c4882926-4882672 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT158933 WP_000356397.1 NZ_CP054191:c4686839-4686549 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT158933 NZ_CP069890:c4883174-4882923 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7