Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 584262..585061 Replicon chromosome
Accession NZ_CP054191
Organism Escherichia coli strain TH9F11

Toxin (Protein)


Gene name yhaV Uniprot ID F4VJD3
Locus tag HRL54_RS02845 Protein ID WP_000347266.1
Coordinates 584262..584726 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag HRL54_RS02850 Protein ID WP_001307405.1
Coordinates 584726..585061 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRL54_RS02815 (579263) 579263..579697 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
HRL54_RS02820 (579715) 579715..580593 - 879 WP_001315856.1 PTS N-acetylgalactosamine transporter subunit IID -
HRL54_RS02825 (580583) 580583..581362 - 780 WP_000406214.1 PTS N-acetylgalactosamine transporter subunit IIC -
HRL54_RS02830 (581373) 581373..581846 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
HRL54_RS02835 (581869) 581869..583149 - 1281 WP_000681921.1 tagatose-bisphosphate aldolase subunit KbaZ -
HRL54_RS02840 (583398) 583398..584207 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
HRL54_RS02845 (584262) 584262..584726 - 465 WP_000347266.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
HRL54_RS02850 (584726) 584726..585061 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
HRL54_RS02855 (585210) 585210..586781 - 1572 WP_001273741.1 galactarate dehydratase -
HRL54_RS02860 (587156) 587156..588490 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
HRL54_RS02865 (588506) 588506..589276 + 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 584262..595717 11455


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17841.18 Da        Isoelectric Point: 9.4947

>T158911 WP_000347266.1 NZ_CP054191:c584726-584262 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRFFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T158911 NZ_CP069883:120164-120439 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATTTGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT158911 WP_001307405.1 NZ_CP054191:c585061-584726 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT158911 NZ_CP069883:119880-120164 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A836NGD2

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References