Detailed information of TA system TA158903

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 51773..52043
• Replicon: plasmid pIncFII
• Accession: NZ_CP054171
• Organism: Klebsiella pneumoniae strain BA12818

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: HBJ12_RS26260
• Protein ID: WP_001312861.1
• Coordinates: 51885..52043 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	51773..51836 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HBJ12_RS26235	47484..48011	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
HBJ12_RS26240	48069..48302	+	234	WP_000006003.1	DUF905 family protein	-
HBJ12_RS26245	48363..50386	+	2024	Protein_60	ParB/RepB/Spo0J family partition protein	-
HBJ12_RS26250	50455..50889	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
HBJ12_RS26255	50886..51605	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	51617..51841	+	225	NuclAT_0	-	-
-	51617..51841	+	225	NuclAT_0	-	-
-	51617..51841	+	225	NuclAT_0	-	-
-	51617..51841	+	225	NuclAT_0	-	-
-	51773..51836	-	64	-	-	Antitoxin
HBJ12_RS26260	51885..52043	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
HBJ12_RS26265	52281..52658	-	378	Protein_64	hypothetical protein	-
HBJ12_RS26270	52958..53254	+	297	WP_001272251.1	hypothetical protein	-
HBJ12_RS26275	53365..54186	+	822	WP_001234445.1	DUF945 domain-containing protein	-
HBJ12_RS26280	54483..55085	-	603	WP_000243713.1	transglycosylase SLT domain-containing protein	-
HBJ12_RS26285	55408..55791	+	384	WP_001354030.1	relaxosome protein TraM	-
HBJ12_RS26290	55985..56656	+	672	WP_000283561.1	conjugal transfer transcriptional regulator TraJ	-
HBJ12_RS26295	56793..57020	+	228	WP_000089263.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / qacE / sul1 / blaNDM-5 / rmtB / blaTEM-1B / mph(A)	-	1..92972	92972

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T158903 WP_001312861.1 NZ_CP054171:51885-52043 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T158903 NZ_CP069882:c3575051-3573729 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACTGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Nucleotide

• Length: 64 bp

>AT158903 NZ_CP054171:c51836-51773 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin