Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-RelB
Location 324393..324979 Replicon chromosome
Accession NZ_CP054170
Organism Klebsiella pneumoniae strain BA12818

Toxin (Protein)


Gene name doc Uniprot ID W8VD46
Locus tag HBJ12_RS01505 Protein ID WP_002920800.1
Coordinates 324611..324979 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID A0A0H3GZM4
Locus tag HBJ12_RS01500 Protein ID WP_002920802.1
Coordinates 324393..324614 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HBJ12_RS01480 320550..321476 + 927 WP_002920807.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
HBJ12_RS01485 321473..322750 + 1278 WP_002920806.1 branched chain amino acid ABC transporter permease LivM -
HBJ12_RS01490 322747..323514 + 768 WP_002920803.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -
HBJ12_RS01495 323516..324229 + 714 WP_075583794.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF -
HBJ12_RS01500 324393..324614 + 222 WP_002920802.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
HBJ12_RS01505 324611..324979 + 369 WP_002920800.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
HBJ12_RS01510 325252..326568 + 1317 WP_002920796.1 sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB -
HBJ12_RS01515 326675..327562 + 888 WP_002920792.1 sn-glycerol-3-phosphate ABC transporter permease UgpA -
HBJ12_RS01520 327559..328404 + 846 WP_002920789.1 sn-glycerol-3-phosphate ABC transporter permease UgpE -
HBJ12_RS01525 328406..329476 + 1071 WP_002920787.1 sn-glycerol-3-phosphate import ATP-binding protein UgpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 321473..330213 8740


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Fido domain

(13-86)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-49)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13552.92 Da        Isoelectric Point: 8.6410

>T158885 WP_002920800.1 NZ_CP054170:324611-324979 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Download         Length: 369 bp

>T158885 NZ_CP069873:c4960901-4960799 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8181.18 Da        Isoelectric Point: 4.7769

>AT158885 WP_002920802.1 NZ_CP054170:324393-324614 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Download         Length: 222 bp

>AT158885 NZ_CP069873:4960792-4960937 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GUD1

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZM4

References