Detailed information of TA system TA158882

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4522516..4523118
• Replicon: chromosome
• Accession: NZ_CP054169
• Organism: Escherichia coli strain STO_Bone1B

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HRS04_RS22810
• Protein ID: WP_000897305.1
• Coordinates: 4522516..4522827 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRS04_RS22815
• Protein ID: WP_000356397.1
• Coordinates: 4522828..4523118 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS04_RS22785 (4518430)	4518430..4519029	+	600	WP_086732335.1	glucose-1-phosphatase	-
HRS04_RS22790 (4519023)	4519023..4519895	+	873	WP_000920762.1	virulence factor BrkB family protein	-
HRS04_RS22795 (4519892)	4519892..4520329	+	438	WP_000560983.1	D-aminoacyl-tRNA deacylase	-
HRS04_RS22800 (4520374)	4520374..4521315	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
HRS04_RS22805 (4521379)	4521379..4522287	-	909	WP_001162704.1	alpha/beta hydrolase	-
HRS04_RS22810 (4522516)	4522516..4522827	+	312	WP_000897305.1	hypothetical protein	Toxin
HRS04_RS22815 (4522828)	4522828..4523118	+	291	WP_000356397.1	NadS family protein	Antitoxin
HRS04_RS22820 (4523723)	4523723..4523941	+	219	WP_001295676.1	CopG family transcriptional regulator	-
HRS04_RS22825 (4524160)	4524160..4524402	+	243	WP_001086388.1	protein YiiF	-
HRS04_RS22830 (4524732)	4524732..4525661	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HRS04_RS22835 (4525658)	4525658..4526293	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HRS04_RS22840 (4526290)	4526290..4527192	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T158882 WP_000897305.1 NZ_CP054169:4522516-4522827 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T158882 NZ_CP069873:c2961953-2961735 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT158882 WP_000356397.1 NZ_CP054169:4522828-4523118 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT158882 NZ_CP069873:c2962354-2961980 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7