Detailed information of TA system TA158847

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 99366..99787
• Replicon: plasmid pBone1BIncF
• Accession: NZ_CP054166
• Organism: Escherichia coli strain STO_Bone1B

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: HRS04_RS00585
• Protein ID: WP_096937776.1
• Coordinates: 99366..99491 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	99589..99787 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS04_RS00545 (94430)	94430..94645	-	216	WP_001352842.1	conjugal transfer relaxosome protein TraY	-
HRS04_RS00550 (94781)	94781..95428	-	648	WP_000332520.1	conjugal transfer transcriptional regulator TraJ	-
HRS04_RS00555 (95619)	95619..96002	-	384	WP_001063020.1	conjugal transfer relaxosome DNA-binding protein TraM	-
HRS04_RS00560 (96372)	96372..96944	+	573	WP_000949660.1	transglycosylase SLT domain-containing protein	-
HRS04_RS00565 (97240)	97240..98061	-	822	WP_001234465.1	DUF932 domain-containing protein	-
HRS04_RS00570 (98180)	98180..98467	-	288	WP_000107242.1	hypothetical protein	-
HRS04_RS00575 (98492)	98492..98698	-	207	WP_000275854.1	hypothetical protein	-
HRS04_RS00580 (98768)	98768..99065	+	298	Protein_115	hypothetical protein	-
HRS04_RS00585 (99366)	99366..99491	-	126	WP_096937776.1	type I toxin-antitoxin system Hok family toxin	Toxin
HRS04_RS00590 (99433)	99433..99582	-	150	Protein_117	DUF5431 family protein	-
- (99589)	99589..99787	-	199	NuclAT_0	-	Antitoxin
- (99589)	99589..99787	-	199	NuclAT_0	-	Antitoxin
- (99589)	99589..99787	-	199	NuclAT_0	-	Antitoxin
- (99589)	99589..99787	-	199	NuclAT_0	-	Antitoxin
HRS04_RS00595 (99756)	99756..99861	-	106	Protein_118	plasmid SOS inhibition protein A	-
HRS04_RS00600 (100016)	100016..100333	+	318	Protein_119	IS110 family transposase	-
HRS04_RS00605 (100622)	100622..100873	-	252	Protein_120	iron-containing alcohol dehydrogenase	-
HRS04_RS00610 (100958)	100958..101470	-	513	WP_223350513.1	potassium channel family protein	-
HRS04_RS00615 (101597)	101597..102290	-	694	Protein_122	IS1 family transposase	-
HRS04_RS00620 (102347)	102347..102451	+	105	Protein_123	thiosulfate reductase cytochrome B subunit	-
HRS04_RS00625 (102697)	102697..104091	-	1395	WP_001037799.1	carbohydrate porin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..165243	165243
	-	flank	IS/Tn	-	-	101597..101974	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4806.72 Da
• Isoelectric Point: 8.4890

>T158847 WP_096937776.1 NZ_CP054166:c99491-99366 [Escherichia coli]
VLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T158847 NZ_CP069865:c105280-105177 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Nucleotide

• Length: 199 bp

>AT158847 NZ_CP054166:c99787-99589 [Escherichia coli]
TCACACAGATTACCCGTAAACAGTCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin