Detailed information of TA system TA158845

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 62244..62498
• Replicon: plasmid pBone1BIncF
• Accession: NZ_CP054166
• Organism: Escherichia coli strain STO_Bone1B

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: HRS04_RS00370
• Protein ID: WP_001312851.1
• Coordinates: 62244..62393 (-)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	62437..62498 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS04_RS00340 (57491)	57491..58402	-	912	WP_000440183.1	carbamate kinase	-
HRS04_RS00345 (58413)	58413..59633	-	1221	WP_000410951.1	arginine deiminase	-
HRS04_RS00350 (60367)	60367..60954	+	588	Protein_69	VENN motif pre-toxin domain-containing protein	-
HRS04_RS00355 (60954)	60954..61400	-	447	Protein_70	plasmid replication initiator RepA	-
HRS04_RS00360 (61393)	61393..61467	-	75	WP_001365705.1	RepA leader peptide Tap	-
HRS04_RS00365 (61703)	61703..61960	-	258	WP_000083833.1	replication regulatory protein RepA	-
HRS04_RS00370 (62244)	62244..62393	-	150	WP_001312851.1	Hok/Gef family protein	Toxin
- (62437)	62437..62498	+	62	NuclAT_1	-	Antitoxin
- (62437)	62437..62498	+	62	NuclAT_1	-	Antitoxin
- (62437)	62437..62498	+	62	NuclAT_1	-	Antitoxin
- (62437)	62437..62498	+	62	NuclAT_1	-	Antitoxin
HRS04_RS00375 (62637)	62637..62819	-	183	WP_000968309.1	hypothetical protein	-
HRS04_RS00380 (62920)	62920..63622	+	703	Protein_75	IS1 family transposase	-
HRS04_RS00385 (63923)	63923..64135	-	213	WP_005012601.1	hypothetical protein	-
HRS04_RS00390 (64268)	64268..64828	-	561	WP_000139363.1	fertility inhibition protein FinO	-
HRS04_RS00395 (64883)	64883..65629	-	747	WP_000205701.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(3)-IIa / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr / tet(A)	-	1..165243	165243
	-	flank	IS/Tn	-	-	63236..63622	386

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T158845 WP_001312851.1 NZ_CP054166:c62393-62244 [Escherichia coli]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T158845 NZ_CP069863:200221-200703 [Klebsiella pneumoniae]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCGAAGGTTTCTATGCTCACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Nucleotide

• Length: 62 bp

>AT158845 NZ_CP054166:62437-62498 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin