Detailed information of TA system TA158841

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 18888..19127
• Replicon: plasmid pBone2IncF
• Accession: NZ_CP054165
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: HRS05_RS25350
• Protein ID: WP_023144756.1
• Coordinates: 18888..19022 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	19067..19127 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS25310 (14235)	14235..14650	-	416	Protein_21	IS1-like element IS1B family transposase	-
HRS05_RS25315 (14899)	14899..15300	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
HRS05_RS25320 (15233)	15233..15490	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
HRS05_RS25325 (15583)	15583..16236	-	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
HRS05_RS25330 (17176)	17176..18033	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
HRS05_RS25335 (18026)	18026..18100	-	75	WP_031943482.1	RepA leader peptide Tap	-
HRS05_RS25340 (18100)	18100..18231	-	132	Protein_27	protein CopA/IncA	-
HRS05_RS25345 (18337)	18337..18591	-	255	WP_000083850.1	replication regulatory protein RepA	-
HRS05_RS25350 (18888)	18888..19022	-	135	WP_023144756.1	Hok/Gef family protein	Toxin
- (19067)	19067..19127	+	61	NuclAT_1	-	Antitoxin
- (19067)	19067..19127	+	61	NuclAT_1	-	Antitoxin
- (19067)	19067..19127	+	61	NuclAT_1	-	Antitoxin
- (19067)	19067..19127	+	61	NuclAT_1	-	Antitoxin
HRS05_RS25355 (19094)	19094..19380	-	287	Protein_30	DUF2726 domain-containing protein	-
HRS05_RS25360 (19893)	19893..20105	-	213	WP_013023861.1	hypothetical protein	-
HRS05_RS25365 (20236)	20236..20796	-	561	WP_042002551.1	fertility inhibition protein FinO	-
HRS05_RS25370 (20851)	20851..21597	-	747	WP_023153769.1	conjugal transfer pilus acetylase TraX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	catB3 / blaOXA-1 / aac(6')-Ib-cr / aadA5 / qacE / sul1	-	1..68411	68411

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T158841 WP_023144756.1 NZ_CP054165:c19022-18888 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T158841 NZ_CP069861:3811822-3813144 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGAGGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGGTTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCTATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Nucleotide

• Length: 61 bp

>AT158841 NZ_CP054165:19067-19127 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin