Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | yafN-yafO (relBE)/YafO-YafN |
| Location | 5097046..5097740 | Replicon | chromosome |
| Accession | NZ_CP054161 | ||
| Organism | Escherichia coli strain STO_Bone2 | ||
Toxin (Protein)
| Gene name | yafO | Uniprot ID | A0A0D8WHS4 |
| Locus tag | HRS05_RS24470 | Protein ID | WP_001521903.1 |
| Coordinates | 5097046..5097444 (-) | Length | 133 a.a. |
Antitoxin (Protein)
| Gene name | yafN | Uniprot ID | S1QAE3 |
| Locus tag | HRS05_RS24475 | Protein ID | WP_000554758.1 |
| Coordinates | 5097447..5097740 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| - (5092876) | 5092876..5092956 | - | 81 | NuclAT_11 | - | - |
| - (5092876) | 5092876..5092956 | - | 81 | NuclAT_11 | - | - |
| - (5092876) | 5092876..5092956 | - | 81 | NuclAT_11 | - | - |
| - (5092876) | 5092876..5092956 | - | 81 | NuclAT_11 | - | - |
| HRS05_RS24440 (5092216) | 5092216..5093460 | - | 1245 | WP_000189541.1 | esterase FrsA | - |
| HRS05_RS24445 (5093552) | 5093552..5094010 | - | 459 | WP_001291990.1 | xanthine phosphoribosyltransferase | - |
| HRS05_RS24450 (5094271) | 5094271..5095728 | + | 1458 | WP_001293009.1 | cytosol nonspecific dipeptidase | - |
| HRS05_RS24455 (5095785) | 5095785..5096137 | - | 353 | Protein_4772 | peptide chain release factor H | - |
| HRS05_RS24460 (5096133) | 5096133..5096339 | - | 207 | Protein_4773 | RtcB family protein | - |
| HRS05_RS24465 (5096584) | 5096584..5097036 | - | 453 | WP_023144376.1 | GNAT family N-acetyltransferase | - |
| HRS05_RS24470 (5097046) | 5097046..5097444 | - | 399 | WP_001521903.1 | type II toxin-antitoxin system mRNA interferase toxin YafO | Toxin |
| HRS05_RS24475 (5097447) | 5097447..5097740 | - | 294 | WP_000554758.1 | type I toxin-antitoxin system antitoxin YafN | Antitoxin |
| HRS05_RS24480 (5097792) | 5097792..5098847 | - | 1056 | WP_001226177.1 | DNA polymerase IV | - |
| HRS05_RS24485 (5098918) | 5098918..5099703 | - | 786 | WP_000207556.1 | putative lateral flagellar export/assembly protein LafU | - |
| HRS05_RS24490 (5099675) | 5099675..5101387 | + | 1713 | Protein_4779 | flagellar biosynthesis protein FlhA | - |
| HRS05_RS24495 (5101466) | 5101466..5101819 | + | 354 | WP_024166466.1 | type II toxin-antitoxin system HicB family antitoxin | - |
| HRS05_RS24500 (5101876) | 5101876..5102625 | - | 750 | WP_016233951.1 | C40 family peptidase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Genomic island | - | - | 5086983..5097740 | 10757 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 133 a.a. Molecular weight: 15471.88 Da Isoelectric Point: 8.0949
>T158840 WP_001521903.1 NZ_CP054161:c5097444-5097046 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF
Download Length: 399 bp
>T158840 NZ_CP069861:c2463429-2463211 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 11233.76 Da Isoelectric Point: 5.2170
>AT158840 WP_000554758.1 NZ_CP054161:c5097740-5097447 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE
Download Length: 294 bp
>AT158840 NZ_CP069861:c2463830-2463456 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0D8WHS4 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | S1QAE3 |