Detailed information of TA system TA158820

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 2103778..2104505
• Replicon: chromosome
• Accession: NZ_CP054161
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A829L2T9
• Locus tag: HRS05_RS09985
• Protein ID: WP_001521139.1
• Coordinates: 2103778..2104089 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRS05_RS09990
• Protein ID: WP_000126294.1
• Coordinates: 2104086..2104505 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS09960 (2099713)	2099713..2101422	+	1710	WP_001521140.1	formate hydrogenlyase subunit HycE	-
HRS05_RS09965 (2101432)	2101432..2101974	+	543	WP_000493785.1	formate hydrogenlyase subunit HycF	-
HRS05_RS09970 (2101974)	2101974..2102741	+	768	WP_000067399.1	formate hydrogenlyase subunit HycG	-
HRS05_RS09975 (2102738)	2102738..2103148	+	411	WP_001291918.1	formate hydrogenlyase assembly protein HycH	-
HRS05_RS09980 (2103141)	2103141..2103611	+	471	WP_000132961.1	hydrogenase maturation peptidase HycI	-
HRS05_RS09985 (2103778)	2103778..2104089	+	312	WP_001521139.1	type II toxin-antitoxin system HigB family toxin	Toxin
HRS05_RS09990 (2104086)	2104086..2104505	+	420	WP_000126294.1	helix-turn-helix domain-containing protein	Antitoxin
HRS05_RS09995 (2104584)	2104584..2106008	-	1425	WP_020234028.1	6-phospho-beta-glucosidase AscB	-
HRS05_RS10000 (2106017)	2106017..2107474	-	1458	WP_020234027.1	PTS cellobiose/arbutin/salicin transporter subunit IIBC	-
HRS05_RS10005 (2107734)	2107734..2108744	+	1011	WP_015912547.1	DNA-binding transcriptional regulator AscG	-
HRS05_RS10010 (2108893)	2108893..2109420	+	528	WP_001078777.1	electron transport protein HydN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12493.23 Da
• Isoelectric Point: 9.4783

>T158820 WP_001521139.1 NZ_CP054161:2103778-2104089 [Escherichia coli]
MHIISKAPFEECARKYPNDALALYSLYRVIKETDFSTPEEMRTAFPNLDNFRYRNKWYVLDVGGNNLRVIAYINFVNKRF
FVKHITNHAEYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T158820 NZ_CP069853:c268447-268163 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15416.42 Da
• Isoelectric Point: 4.4596

>AT158820 WP_000126294.1 NZ_CP054161:2104086-2104505 [Escherichia coli]
MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEEDDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPV
GVALLRTLIDQHNLTYADLKNEIGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT158820 NZ_CP069853:c268679-268437 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Toxin

• AlphaFold DB: A0A829L2T9