Detailed information of TA system TA158815

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 738323..738925
• Replicon: chromosome
• Accession: NZ_CP054161
• Organism: Escherichia coli strain STO_Bone2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: HRS05_RS03495
• Protein ID: WP_000897305.1
• Coordinates: 738614..738925 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: HRS05_RS03490
• Protein ID: WP_000356395.1
• Coordinates: 738323..738613 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRS05_RS03455 (733946)	733946..734848	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
HRS05_RS03460 (734845)	734845..735480	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
HRS05_RS03465 (735477)	735477..736406	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
HRS05_RS03470 (736588)	736588..736830	-	243	WP_021523315.1	hypothetical protein	-
HRS05_RS03475 (737049)	737049..737267	-	219	WP_001295676.1	CopG family transcriptional regulator	-
HRS05_RS03480 (737686)	737686..737964	-	279	WP_001306650.1	hypothetical protein	-
HRS05_RS03485 (738026)	738026..738238	-	213	WP_000197769.1	hypothetical protein	-
HRS05_RS03490 (738323)	738323..738613	-	291	WP_000356395.1	NadS family protein	Antitoxin
HRS05_RS03495 (738614)	738614..738925	-	312	WP_000897305.1	hypothetical protein	Toxin
HRS05_RS03500 (739154)	739154..740062	+	909	WP_001306651.1	alpha/beta hydrolase	-
HRS05_RS03505 (740231)	740231..741145	-	915	WP_077634137.1	transposase	-
HRS05_RS03510 (741158)	741158..742045	-	888	Protein_681	hypothetical protein	-
HRS05_RS03515 (742534)	742534..742992	-	459	WP_000526135.1	IS200/IS605-like element IS200C family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	742534..742992	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T158815 WP_000897305.1 NZ_CP054161:c738925-738614 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T158815 NZ_CP069847:4194468-4194878 [Klebsiella pneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAAATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT158815 WP_000356395.1 NZ_CP054161:c738613-738323 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT158815 NZ_CP069847:4194221-4194487 [Klebsiella pneumoniae]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7